National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A soil isolate of Bacillus cereus exhibited highest proteolytic activity when grown on casein agar plates with a zone of inhibition of 34mm. The production medium was optimized with respect to physical and chemical factors using submerged fermentation. The optimum pH, temperature and incubation period for protease production were pH 7 at 37°C for 72 hrs at 150rpm. Glucose was found to be an important media component in this. The protease production of this isolate was also studied with respect to the inoculum size, nitrogen sources and metal ions. Ca2+ was found to be a potent enhancer. The extracted crude enzyme showed the ability to remove blood stains and so can be used in detergent industry.
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