Development and Validation of an HPLC-PDA Method for Quantitation of Ten Marker Compounds from Eclipta prostrata (L.) and Evaluation of Their Protein Tyrosine Phosphatase 1B, α-Glucosidase, and Acetylcholinesterase Inhibitory Activities

Duc Hung Nguyen; Duc Dat Le; Eun Sook Ma; Byung Sun Min; Mi Hee Woo

doi:10.20307/nps.2020.26.4.326

주제분류

...

저널정보

한국생약학회
Natural Product Sciences 학술저널
Natural Product Sciences Vol.26 No.4
2020.12 326 - 333 (8page)
DOI : 10.20307/nps.2020.26.4.326

저자정보

Duc Hung Nguyen (Catholic University of Daegu)
Duc Dat Le (Catholic University of Daegu)
Eun Sook Ma (Catholic University of Daegu)
Byung Sun Min (Catholic University of Daegu)
Mi Hee Woo (Catholic University of Daegu)

이용수
내서재: 0

내서재에 추가
되었습니다. 내서재에서
삭제되었습니다.

초록·키워드

오류제보하기

The aerial parts of Eclipta prostrata is used as a traditional medicine and vegetable. In traditional folk medicine, it is used for treatment of hemorrhages, hepatic, disease, renal injuries, hair loss, tooth mobility, and viper bites. In this study, ten compounds (1 ‒ 10) were isolated from the aerial parts of E. prostrata. A reliable high performance liquid chromatography equipped with photometric diode array detector (HPLC-PDA) method was developed to simultaneously quantitate 10 marker compounds [chlorogenic acid (1), paratensein 7-O-β-ᴅ-glucoside (2), quercetin 7-O-β-ᴅ-glucoside (3), luteolin 7-O-β-ᴅ-glucoside (4), apigenin 7-O-β-ᴅ-glucoside (5), apigenin 4"-O-β-ᴅ-glucoside (6), apigenin (7), luteolin (8), wedelolactone (9), and paratensein (10)]. In addition, compounds 5 and 6 showed considerable inhibitory effects against protein-tyrosine phosphatase 1B (PTP1B) enzyme. Moreover, compounds 6 ‒ 8, and 10 exhibited potent α-glucosidase inhibitory effects with IC₅₀ values of 24.5 ± 1.9, 33.0 ± 0.5, 45.5 ± 0.1, and 23.8 ± 1.0 μM, respectively. All compounds (1 ‒ 10) showed considerable acetylcholinesterase (AChE) inhibitory effects with IC₅₀ ranging from 30.1 to 75.2 μM.

#Eclipta prostrata #PTP1B #α-Glucosidase #AChE inhibitory activity