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Molecular characteristics and expression pattern of miR167 family and their potential targets during early somatic embryogenesis in longan(Dimocarpus longan Lour.)Chinese Full TextEnglish Full Text (MT)

WANG Jingyu;SHEN Xu;CHEN Xiaohui;XU Xiaoping;ZHANG Shuting;Su Liyao;LIN Yuling;LAI Zhongxiong;Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University;

Abstract: To understand the molecular characteristics of members of the miR167 family and the regulatory mechanism of their potential targets during early somatic embryogenesis in longan trees(Dimocarpus longan Lour.), bioinformatics analysis was used to analyze the sequences of the longan miR167 gene family, secondary structure, molecular evolutionary tree, target gene prediction, and the specific expression of FPKM of their targets. Quantitative real-time PCR(qPCR) was also used to detect the transcription level of the miR167 family during early somatic embryogenesis. The results showed that the longan miR167 precursor sequences had an overlapping region with a length of 20 nt. In addition, there were multiple base differences between the middle sequence region and the 3’ end of miR167b and this region. All the members of longan miR167 precursors can form stem ring structures, and the minimum folding free energy was between-79.95 Kal/mol and-46.90 Kal/mol. The molecular evolutionary tree showed that the longan miR167 precursor members were most closely related to Carica papaya, Solanum lycopersicum, Sorghum bicolor, and Glycine max, respectively, miR167 a, c, d, and f converged on the same branch in the mature evolutionary tree. Target prediction results showed that they might regulate six identical target genes, namely E3 ubiquitin ligase BIG BROTHER(BB), AIRP2, MYB transcription factor, and auxin response factor(ARF6, ARF8, ARF6-3). miR167 b was isolated in another branch and might regulate two specific targets, lysine-specific histone demethylase-1(LSD1) and cyclin(cyclin-c1-2-like). The results of qPCR showed that the expression of dlo-miR167 a, c, and d was upregulated, and the expression levels of dlo-miR167 d and f in the three stages were low. The FPKM values showed that the expression patterns of ARF6/8 and BB during early somatic embryogenesis were consistent with dlo-miR167 a, c and d, and negatively correlated with AIRP2. This study suggested that ARF6/8 and BB might not be targeted by miR167 during early somatic embryogenesis, and that miR167 may participate in the morphogenesis of early somatic embryogenesis by targeting AIRP2, suggesting that synergism and independence of the miR167 gene function coexist.
  • DOI:

    10.19675/j.cnki.1006-687x.2020.01049

  • Series:

    (A) Mathematics/ Physics/ Mechanics/ Astronomy; (B) Chemistry/ Metallurgy/ Environment/ Mine Industry; (D) Agriculture

  • Subject:

    Horticulture

  • Classification Code:

    S667.2

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