BOOKSHOP

Type: Chapter

Advances in guide RNA design for editing plant genomes using CRISPR-Cas systems

Authors

Kaiyuan Chen

Huazhong Agricultural University (China)

Hao Liu

Huazhong Agricultural University (China)

Kabin Xie

Huazhong Agricultural University (China)

Muhammad Tahir ul Qamar

Huazhong Agricultural University (China)

Ling-Ling Chen

Huazhong Agricultural University (China)

Publication date:

19 April 2021

ID: 9781801461382

E-Chapter format

£25.00
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Description

This chapter discusses the general rules for selecting target sites for genome editing using the CRISPR-Cas technology and summarizes the bioinformatic tools that can be used to design sgRNA sequences.

Table of contents

1 Introduction 2 Designing sgRNAs 3 Bioinformatics programs for designing sgRNAs 4 Predicting the outcome of CRISPR-mediated genome editing 5 Conclusion 6 Where to look for further information 7 References