DOI : https://doi.org/10.18517/ijaseit.10.1.9770

The Characterization of Ficus lyrata Warb Fruit Extract and the Effect on Toxicity, Physicochemical, and Microbiology Properties of Chicken Carcass

Dwi Wahyudha Wira (1), Efri Mardawati (2), Mohamad Djali (3), Roostira Lobo Balia (4)
(1) Study Programme of Veterinary Medicine, Faculty of Medicine, Universitas Padjadjaran, Km 21 Jatinangor, 45363, Indonesia
(2) Department of Agroindustrial Technology, Faculty of Agroindustrial Technology, Universitas Padjadjaran, Km 21 Jatinangor, 45363, Indonesia
(3) Department of Agroindustrial Technology, Faculty of Agroindustrial Technology, Universitas Padjadjaran, Km 21 Jatinangor, 45363, Indonesia
(4) Study Programme of Veterinary Medicine, Faculty of Medicine, Universitas Padjadjaran, Km 21 Jatinangor, 45363, Indonesia
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How to cite (IJASEIT) :
Wira, Dwi Wahyudha, et al. “The Characterization of Ficus Lyrata Warb Fruit Extract and the Effect on Toxicity, Physicochemical, and Microbiology Properties of Chicken Carcass”. International Journal on Advanced Science, Engineering and Information Technology, vol. 10, no. 1, Feb. 2020, pp. 362-7, doi:10.18517/ijaseit.10.1.9770.
Citation Format :
The use of plant extraction as natural and safe preservatives, have been highly recommended for the improvement of the microbiological and sensory quality of food.  In the present investigation, the dried fruit of Ficus lyrata Warb was subjected to bio preservative to chicken carcass. This study aimed to characterize the ethyl acetate extract on the phytochemical compound, antioxidant activity, antibacterial activity, to determine the toxicity of the extraction with lethal dose (LD50) value and to study the application of the extract as chicken carcass preservative. The result showed that the fruit extract has phenolic compounds, flavonoids and tannins. Gas Chromatography Analysis resulted that the extract contains catechin, luteolin-6.8-C-diglukoside and Orientin.  DPPH and clear zone analysis showed that the extract has a high of antioxidant and antibacterial activity. The acute toxicity analyzes showed that the extract of Ficus lyrata Warb fruit has not the LD50 below the concentration of 15000 mg/kg weight, that means the extract was practically non-toxic material. The application of extract to chicken carcass preservation had resulted the pH of carcass in the range 5.51—5.98 and decreased the temperature from 26 to 24oC and effected to inhibit of chicken carcass decay for 2 hours.  The extract of F. lyrata Warb fruit also inhibited the bacterial growth until 2.4 x 106 CFU/g within the 12 hours of storage and increased the tenderness of carcass to 0.6 N. Based on the observed characteristics, it was obtained that the use fruit extract as a natural preservative can extend the shelf life of fresh chicken carcasses.

M. Jagathambal, K. Madhavan, V. Parvathi, and G. Karunakaran, “Phytochemical screening and antimicrobial activity of seven solvent extracts of dried fruit of Ficus carica against five human pathogenic bacteria Phytochemical screening and antimicrobial activity of seven solvent extracts of dried fruit of Ficus carica ,” no. August, 2014.

V. R. Mohan, A. Nishanthini, and G. S. Devi, “Evaluation of Antioxidant Properties of Ficus bengalensis Bark Evaluation of Antioxidant Properties of Ficus bengalensis Bark .,” no. August, 2015.

E. A. Adebayo, O. R. Ishola, O. S. Taiwo, O. N. Majolagbe, and B. T. Adekeye, “Evaluations of the methanol extract of Ficus exasperata stem bark , leaf and root for phytochemical analysis and antimicrobial activities,” vol. 3, no. 12, pp. 283-287, 2009.

S. Bidarigh, K. P. M. R. M, and M. A. I. Kh, “In vitro anti-Candida activity of Ficus lyrata L . Ethyl acetate latex extract and Nystatin on clinical Isolates and Standard strains of Candida albicans,” vol. 18, pp. 115-119, 2011.

A. Kumar, V. Tomer, and Y. Gat, “Ficus religiosa : A wholesome medicinal tree Ficus religiosa : A wholesome medicinal tree,” no. July, 2018.

D. W. Wira, M. Djali, and R. Balia, “The study of potent and phtochemical on fruit extract of Ficus lyrata Warb as biopreservative,” study potent phtochemical fruit Extr. Ficus lyrata Warb as biopreservative, vol. 8, 2014.

H. Sun, T. Mu, and L. Xi, “Effect of pH , heat , and light treatments on the antioxidant activity of sweet potato leaf polyphenols,” Int. J. Food Prop., vol. 20, no. 2, pp. 318-332, 2017.

F. Toledo et al., “Screening of the antioxidant and nutritional properties , phenolic contents and proteins of five durian cultivars,” vol. 59, no. August, 2008.

L. Zhang, Y. Xu, Y. Li, T. Bao, V. Gowd, and W. Chen, “Protective property of mulberry digest against oxidative stress - A potential approach to ameliorate dietary acrylamide-induced cytotoxicity,” Food Chem., vol. 230, pp. 306-315, 2017.

Y. Zhang, Y. Wan, and B. Huo, “Extracts and components of Ficus carica leaves suppress survival , cell cycle , and migration of triple-negative breast cancer MDA-MB-231 cells,” pp. 4377-4386, 2018.

M. W. Mumtaz et al., “Metabolite profiling and inhibitory properties of leaf extracts of Ficus benjamina towards α - glucosidase and α -amylase,” Int. J. Food Prop., vol. 21, no. 1, pp. 1560-1574, 2018.

I. I. Koleva, T. A. Van Beek, J. P. H. Linssen, A. De Groot, and L. N. Evstatieva, “Screening of Plant Extracts for Antioxidant Activity : a Comparative Study on Three Testing Methods,” vol. 17, no. June 2001, pp. 8-17, 2002.

P. Molyneux, “The use of the stable free radical diphenylpicryl- hydrazyl ( DPPH ) for estimating antioxidant activity,” vol. 50, no. June 2003.

M. Djali, E. Mardawati, H. Marta, D. W. Wira, K. R. Ramadhani, and R. L. Balia, “The Effect of Ficus lyrata Warb Leaf Extract on Physicochemical and Microbiological Properties of Chicken Carcass,” vol. 9, no. 4, pp. 1409-1413, 2019.

I. O. P. C. Series and M. Science, “The comparative study of the fruit and leaf extract of Ficuslyrata Warb on antibacterial activities,” 2018.

V. Tambe and R. Bhambar, “Phytochemical screening and anthelmintic activity of wood and leaves of hibiscus tiliaceus linn .,” vol. 3, no. 10, pp. 880-889, 2014.

T. Flavors and P. B. Eber-, “Book Re V iews,” pp. 711-713, 2007.

F. Toledo and P. Arancibia-avila, “Screening of the antioxidant and nutritional properties , phenolic contents and proteins of five durian cultivars Screening of the antioxidant and nutritional properties , phenolic contents and proteins of five durian cultivars,” no. June 2014, 2008.

Hodgon and A. Ernest., A Textbook of modern toxicology. 2rd ed. McGraw-hill Book Co. Singapore. Singapore.

T. A.Loomi. and A. W. Hayes, Essential of Toxicology. 1996.

S. A. Woodward and A. Sciences, “Marketing And Products Texture of Cooked Egg Yolk as Influenced by Physical Manipulation of Raw Egg Yolk and Salt Brining of Shell Eggs 1,” pp. 1264-1268, 1987.

M. .Prakash, A., Rigelhof, F., Antioxidant Activity, 10th ed., no. 4. .

K. A. Buckle, R. A. Edward, G. H. Fleet, and M. Wootton., “Food science,” 2007.

R. A. Lawrie, Meat science. Jakarta: UI Press, 2003.

S. Perry and R. H. Perry, Chemical Engineers ’ Handbook Seventh Late Editor.

I. M Smallwood., Handbook of Organic Solvent Properties. London, 1996.

V. P. Bintoro, “Dengan Memakai Uji Fisiko Kimia Dan Mikrobiologi ( The Comparison of the Slaughtered and Nonslaughtered Chicken Meat Using Physico-chemical and Microbiological Test ),” pp. 259-267.

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