An nMgO containing scaffold: Antibacterial activity, degradation properties and cell responses

Authors

  • Cijun Shuai State Key Laboratory of High Performance Complex Manufacturing, College of Mechanical and Electrical Engineering, Central South University, Changsha, China;Jiangxi University of Science and Technology, Ganzhou, China; Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Changsha, China
  • Wang Guo State Key Laboratory of High Performance Complex Manufacturing, College of Mechanical and Electrical Engineering, Central South University, Changsha, China
  • Chengde Gao State Key Laboratory of High Performance Complex Manufacturing, College of Mechanical and Electrical Engineering, Central South University, Changsha, China
  • Youwen Yang State Key Laboratory of High Performance Complex Manufacturing, College of Mechanical and Electrical Engineering, Central South University, Changsha, China
  • Ping Wu College of Chemistry, Xiangtan University, Xiangtan, China
  • Pei Feng State Key Laboratory of High Performance Complex Manufacturing, College of Mechanical and Electrical Engineering, Central South University, Changsha, China

DOI:

https://doi.org/10.18063/ijb.v4i1.120

Keywords:

Nano magnesium oxide, antibacterial scaffolds, degradation properties, cytocompatibility, mechanical properties

Abstract

Bone repair failure caused by implant-related infections is a common and troublesome problem. In this study, an antibacterial scaffold was developed via selective laser sintering with incorporating nano magnesium oxide (nMgO) to poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). The results indicated the scaffold exerted high antibacterial activity. The antibacterial mechanism was that nMgO could cause oxidative damage and mechanical damage to bacteria through the production of reactive oxygen species (ROS) and direct contact action, respectively, which resulted in the damage of their structures and functions. Besides, nMgO significantly increased the compressive properties of the scaffold including strength and modulus, due to its excellent mechanical properties and uniform dispersion in the PHBV matrix. Moreover, the degradation tests indicated nMgO neutralized the acid degradation products of PHBV and benefited the degradation of the scaffold. The cell culture demonstrated that nMgO promoted the cellular adhesion and proliferation, as well as osteogenic differentiation. The present work may open the door to exploring nMgO as a promising antibacterial material for tissue engineering. 

References

Zimmerli W, 2014, Clinical presentation and treatment of orthopaedic implant-associated infection. J Intern Med, 276(2): 111–119. http://dx.doi.org/10.1111/joim.12233

Saidin S, Chevallier P, Abdul Kadir M R, et al., 2013, Polydopamine as an intermediate layer for silver and hydroxyapatite immobilisation on metallic biomaterials surface. Mater Sci Eng C Mater Biol Appl, 33(8): 4715–4724. http://dx.doi.org/10.1016/j.msec.2013.07.026

Lorenzetti M, Dogsa I, Stosicki T, et al., 2015, The influence of surface modification on bacterial adhesion to titanium-based substrates. ACS Appl Mater Interfaces, 7(3): 1644–1651. http://dx.doi.org/10.1021/am507148n

Overbye K and Barrett J, 2005, Antibiotics: Where did we go wrong? Drug Discov Today, 10(1): 45–52. http://dx.doi.org/10.1016/s1359-6446(04)03285-4

Londonkar R L, Madire Kattegouga U, Shivsharanappa K, et al., 2013, Phytochemical screening and in vitro antimicrobial activity of Typha angustifolia Linn leaves extract against pathogenic gram negative micro organisms. J Pharm Res, 6(2): 280–283. http://dx.doi.org/10.1016/j.jopr.2013.02.010

Trampuz A and Zimmerli W, 2006, Antimicrobial agents in orthopaedic surgery. Drugs, 66(8): 1089–1106. http://dx.doi.org/10.2165/00003495-200666080-00005

Goodman S B, Yao Z, Keeney M, et al., 2013, The future of biologic coatings for orthopaedic implants. Biomaterials, 34(13): 3174–3183. http://dx.doi.org/10.1016/j.biomaterials.2013.01.074

Yang S, Zhang Y, Yu J, et al., 2014, Antibacterial and mechanical properties of honeycomb ceramic materials incorporated with silver and zinc. Mater Des, 59: 461–465. http://dx.doi.org/10.1016/j.matdes.2014.03.025

Yazdimamaghani M, Vashaee D, Assefa S, et al., 2014, Hybrid macroporous gelatin/bioactive-glass/nanosilver scaffolds with controlled degradation behavior and antimicrobial activity for bone tissue engineering. J Biomed Nanotechnol, 10(6): 911–931. http://dx.doi.org/10.1166/jbn.2014.1783

Sánchez-Salcedo S, Shruti S, Salinas A J, et al., 2014, In vitro antibacterial capacity and cytocompatibility of SiO2–CaO–P2O5 meso-macroporous glass scaffolds enriched with ZnO. J Mater Chem B, 2(30): 4836–4847. http://dx.doi.org/10.1039/c4tb00403e

Vargas-Reus M A, Memarzadeh K, Huang J, et al., 2012, Antimicrobial activity of nanoparticulate metal oxides against peri-implantitis pathogens. Int J Antimicrob Agents, 40(2): 135–139. http://dx.doi.org/10.1016/j.ijantimicag.2012.04.012

Dizaj S M, Lotfipour F, Barzegar-Jalali M, et al., 2014, Antimicrobial activity of the metals and metal oxide nanoparticles. Mater Sci Eng C Mater Biol Appl, 44: 278–284. http://dx.doi.org/10.1016/j.msec.2014.08.031

Li Y, Zhang W, Niu J, et al., 2012, Mechanism of photogenerated reactive oxygen species and correlation with the antibacterial properties of engineered metal-oxide nanoparticles. ACS Nano, 6(6): 5164–5173. http://dx.doi.org/10.1021/nn300934k

Krishnamoorthy K, Moon J Y, Hyun H B, et al., 2012, Mechanistic investigation on the toxicity of MgO nanoparticles toward cancer cells. J Mater Chem, 22(47): 24610–24617. http://dx.doi.org/10.1039/c2jm35087d

Staiger M P, Pietak A M, Huadmai J, et al., 2006, Magnesium and its alloys as orthopedic biomaterials: A review. Biomaterials, 27(9): 1728–1734. http://dx.doi.org/10.1016/j.biomaterials.2005.10.003

De Silva R T, Mantilaka M M, Ratnayake S P, et al., 2017, Nano-MgO reinforced chitosan nanocomposites for high performance packaging applications with improved mechanical, thermal and barrier properties. Carbohydr Polym, 157: 739–747. http://dx.doi.org/10.1016/j.carbpol.2016.10.038

Zhao Y, Liu B, You C, et al., 2016, Effects of MgO whiskers on mechanical properties and crystallization behavior of PLLA/MgO composites. Mater Des, 89: 573–581. http://dx.doi.org/10.1016/j.matdes.2015.09.157

Haldorai Y and Shim J-J, 2014, An efficient removal of methyl orange dye from aqueous solution by adsorption onto chitosan/MgO composite: A novel reusable adsorbent. Appl Surf Sci, 292: 447–453. http://dx.doi.org/10.1016/j.apsusc.2013.11.158

Yamamoto O, Ohira T, Alvarez K, et al., 2010, Antibacterial characteristics of CaCO3–MgO composites. Mater Sci Eng B, 173(1–3): 208–212. http://dx.doi.org/10.1016/j.mseb.2009.12.007

Ma F, Lu X, Wang Z, et al., 2011, Nanocomposites of poly(ʟ-lactide) and surface modified magnesia nanoparticles: Fabrication, mechanical property and biodegradability. J Phys Chem Solids, 72(2): 111–116. http://dx.doi.org/10.1016/j.jpcs.2010.11.008

Feng P, Peng S, Wu P, et al., 2016, A space network structure constructed by tetraneedlelike ZnO whiskers supporting boron nitride nanosheets to enhance comprehensive properties of poly (ʟ-lacti acid) scaffolds. Sci Rep, 6: 33385. http://dx.doi.org/10.1038/srep33385

Lee J M, Sing S L, Tan E Y S, et al., 2016, Bioprinting in cardiovascular tissue engineering: A review. Int J Bioprint, 2(2): 27–36. http://dx.doi.org/10.18063/IJB.2016.02.006

Murphy C, Kolan K, Li W, et al., 2017, 3D bioprinting of stem cells and polymer/bioactive glass composite scaffolds for bone tissue engineering. Int J Bioprint, 3(1): 54–64. http://dx.doi.org/10.18063/IJB.2017.01.005

Eshraghi S and Das S, 2010, Mechanical and microstructural properties of polycaprolactone scaffolds with 1-D, 2-D, and 3-D orthogonally oriented porous architectures produced by selective laser sintering. Acta Biomater, 6(7): 2467–2476. http://dx.doi.org/10.1016/j.actbio.2010.02.002

Eshraghi S and Das S, 2012, Micromechanical finite-element modeling and experimental characterization of the compressive mechanical properties of polycaprolactone–hydroxyapatite composite scaffolds prepared by selective laser sintering for bone tissue engineering. Acta Biomater, 8(8): 3138–3143. http://dx.doi.org/10.1016/j.actbio.2012.04.022

Amalric J, Mutin P H, Guerrero G, et al., 2009, Phosphonate monolayers functionalized by silver thiolate species as antibacterial nanocoatings on titanium and stainless steel. J Mater Chem, 19(1): 141–149. http://dx.doi.org/10.1039/b813344a

Simchi A, Tamjid E, Pishbin F, et al., 2011, Recent progress in inorganic and composite coatings with bactericidal capability for orthopaedic applications. Nanomedicine, 7(1): 22–39. http://dx.doi.org/10.1016/j.nano.2010.10.005

Ye L, Liu J, Jiang Z, et al., 2013, Facets coupling of BiOBr-g-C3N4 composite photocatalyst for enhanced visible-light-driven photocatalytic activity. Appl Catal B, 142–143: 1–7. http://dx.doi.org/10.1016/j.apcatb.2013.04.058

Wu D, Wang B, Wang W, et al., 2015, Visible-light-driven BiOBr nanosheets for highly facet-dependent photocatalytic inactivation of Escherichia coli. J Mater Chem A, 3(29): 15148–15155. http://dx.doi.org/10.1039/c5ta02757h

Bruzauskaite I, Bironaite D, Bagdonas E, et al., 2016, Scaffolds and cells for tissue regeneration: Different scaffold pore sizes-different cell effects. Cytotechnology, 68(3): 355–369. http://dx.doi.org/10.1007/s10616-015-9895-4

Roosa S M, Kemppainen J M, Moffitt E N, et al., 2010, The pore size of polycaprolactone scaffolds has limited influence on bone regeneration in an in vivo model. J Biomed Mater Res A, 92(1): 359–368. http://dx.doi.org/10.1002/jbm.a.32381

Schek R M, Wilke E N, Hollister S J, et al., 2006, Combined use of designed scaffolds and adenoviral gene therapy for skeletal tissue engineering. Biomaterials, 27(7): 1160–1166. http://dx.doi.org/10.1016/j.biomaterials.2005.07.029

Ten E, Jiang L and Wolcott M P, 2012, Crystallization kinetics of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/cellulose nanowhiskers composites. Carbohydr Polym, 90(1): 541. http://dx.doi.org/10.1016/j.carbpol.2012.05.076

Shuai C, Guo W, Gao C, et al., 2017, Calcium silicate improved bioactivity and mechanical properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) scaffolds. Polymers, 9(5): 175. http://dx.doi.org/10.3390/polym9050175

Yin Y, Zhang G and Xia Y, 2002, Synthesis and characterization of MgO nanowires through a vapor-phase precursor method. Adv Funct Mater, 12(4): 293–298. http://dx.doi.org/10.1002/1616-3028(20020418)12:4<293::aid-adfm293>3.0.co;2-u

Hutmacher D W, Schantz J T, Lam C X, et al., 2007, State of the art and future directions of scaffold-based bone engineering from a biomaterials perspective. J Tissue Eng Regen Med, 1(4): 245–260. http://dx.doi.org/10.1002/term.24

Ning N-y, Yin Q-j, Luo F, et al., 2007, Crystallization behavior and mechanical properties of polypropylene/halloysite composites. Polymer, 48(25): 7374–7384. http://dx.doi.org/10.1016/j.polymer.2007.10.005

Li H Y, Tan Y Q, Zhang L, et al., 2012, Bio-filler from waste shellfish shell: Preparation, characterization, and its effect on the mechanical properties on polypropylene composites. J Hazard Mater, 217–218: 256–262. http://dx.doi.org/10.1016/j.jhazmat.2012.03.028

He F, Fan J and Lau S, 2008, Thermal, mechanical, and dielectric properties of graphite reinforced poly(vinylidene fluoride) composites. Polym Test, 27(8): 964–970. http://dx.doi.org/10.1016/j.polymertesting.2008.08.010

Maity J, Jacob C, Das C K, et al., 2008, Direct fluorination of Twaron fiber and the mechanical, thermal and crystallization behaviour of short Twaron fiber reinforced polypropylene composites. Compos Part A Appl Sci Manuf, 39(5): 825–833. http://dx.doi.org/10.1016/j.compositesa.2008.01.009

Peng D, Qin W, Wu X, et al., 2015, Improvement of the resistance performance of carbon/cyanate ester composites during vacuum electron radiation by reduced graphene oxide modified TiO2. RSC Adv, 5(94): 77138–77146. http://dx.doi.org/10.1039/c5ra11113g

Liu G, Zhou T, Liu W, et al., 2014, Enhanced desulfurization performance of PDMS membranes by incorporating silver decorated dopamine nanoparticles. J Mater Chem A, 2(32): 12907. http://dx.doi.org/10.1039/c4ta01778a

Lee S-W, Han S M and Nix W D, 2009, Uniaxial compression of fcc Au nanopillars on an MgO substrate: The effects of prestraining and annealing. Acta Mater, 57(15): 4404–4415. http://dx.doi.org/10.1016/j.actamat.2009.06.002

Applerot G, Lellouche J, Lipovsky A, et al., 2012, Understanding the antibacterial mechanism of CuO nanoparticles: Revealing the route of induced oxidative stress. Small, 8(21): 3326–3337. http://dx.doi.org/10.1002/smll.201200772

Applerot G, Lipovsky A, Dror R, et al., 2009, Enhanced antibacterial activity of nanocrystalline ZnO due to increased ROS-mediated cell injury. Adv Funct Mater, 19(6): 842–852. http://dx.doi.org/10.1002/adfm.200801081

Sawai J, Kojima H, Igarashi H, et al., 2000, Antibacterial characteristics of magnesium oxide powder. World J Microbiol Biotechnol, 16(2): 187–194. http://dx.doi.org/10.1023/A:1008916209784

Krishnamoorthy K, Manivannan G, Kim S J, et al., 2012, Antibacterial activity of MgO nanoparticles based on lipid peroxidation by oxygen vacancy. J Nanopart Res, 14(9): 1063. http://dx.doi.org/10.1007/s11051-012-1063-6

Sterrer M, Diwald O and Knözinger E, 2000, Vacancies and electron deficient surface anions on the surface of MgO nanoparticles. J Phys Chem B, 104(15): 3601–3607. http://dx.doi.org/10.1021/jp993924l

Berger T, Sterrer M, Stankic S, et al., 2005, Trapping of photogenerated charges in oxide nanoparticles. Mater Sci Eng C, 25(5–8): 664–668. http://dx.doi.org/10.1016/j.msec.2005.06.013

Sterrer M, Berger T, Diwald O, et al., 2003, Energy transfer on the MgO surface, monitored by UV-induced H2 chemisorption. J Am Chem Soc, 125(1): 195–199. http://dx.doi.org/10.1021/ja028059o

Long T C, Saleh N, Tilton R D, et al., 2006, Titanium dioxide (P25) produces reactive oxygen species in immortalized brain microglia (BV2): Implications for nanoparticle neurotoxicity. Environ Sci Technol, 40(14): 4346–4352. http://dx.doi.org/10.1021/es060589n

Xia T, Kovochich M, Brant J, et al., 2006, Comparison of the abilities of ambient and manufactured nanoparticles to induce cellular toxicity according to an oxidative stress paradigm. Nano Lett, 6(8): 1794–1807. http://dx.doi.org/10.1021/nl061025k

Jin T and He Y, 2011, Antibacterial activities of magnesium oxide (MgO) nanoparticles against foodborne pathogens. J Nanopart Res, 13(12): 6877–6885. http://dx.doi.org/10.1007/s11051-011-0595-5

Yamamoto O, Sawai J, Kojima H, et al., 2002, Effect of mixing ratio on bactericidal action of MgO–CaO powders. J Mater Sci Mater Med, 13(8): 789–792. http://dx.doi.org/10.1023/A:1016179225955

Jeevanandam P and Klabunde K, 2002, A study on adsorption of surfactant molecules on magnesium oxide nanocrystals prepared by an aerogel route. Langmuir, 18(13): 5309–5313. http://dx.doi.org/10.1021/la0200921

He Y, Ingudam S, Reed S, et al., 2016, Study on the mechanism of antibacterial action of magnesium oxide nanoparticles against foodborne pathogens. J Nanobiotechnol, 14(1): 54. http://dx.doi.org/10.1186/s12951-016-0202-0

Salomao R, Bittencourt L and Pandolfelli V, 2007, A novel approach for magnesia hydration assessment in refractory castables. Ceram Int, 33(5): 803–810. http://dx.doi.org/10.1016/j.ceramint.2006.01.004

Mo L, Deng M, Tang M, et al., 2014, MgO expansive cement and concrete in China: Past, present and future. Cem Concr Res, 57: 1–12. http://dx.doi.org/10.1016/j.cemconres.2013.12.007

Shan D, Shi Y, Duan S, et al., 2013, Electrospun magnetic poly (ʟ-lactide) (PLLA) nanofibers by incorporating PLLA-stabilized Fe3O4 nanoparticles. Mater Sci Eng C, 33(6): 3498–3505. http://dx.doi.org/10.1016/j.msec.2013.04.040

Marom R, Shur I, Solomon R, et al., 2005, Characterization of adhesion and differentiation markers of osteogenic marrow stromal cells. J Cell Physiol, 202(1): 41–48. http://dx.doi.org/10.1002/jcp.20109

Wang F, Zhai D, Wu C, et al., 2016, Multifunctional mesoporous bioactive glass/upconversion nanoparticle nanocomposites with strong red emission to monitor drug delivery and stimulate osteogenic differentiation of stem cells. Nano Res, 9(4): 1193–1208. http://dx.doi.org/10.1007/s12274-016-1015-z

Zhang J and Zhu Y, 2014, Synthesis and characterization of CeO2-incorporated mesoporous calcium-silicate materials. Microporous Mesoporous Mater, 197: 244–251. http://dx.doi.org/10.1016/j.micromeso.2014.06.018

Hoppe A, Guldal N S and Boccaccini A R, 2011, A review of the biological response to ionic dissolution products from bioactive glasses and glass-ceramics. Biomaterials, 32(11): 2757–2774. http://dx.doi.org/10.1016/j.biomaterials.2011.01.004

Yamniuk A P and Vogel H J, 2005, Calcium- and magnesium-dependent interactions between calcium- and integrin-binding protein and the integrin αIIb cytoplasmic domain. Protein Sci, 14(6): 1429–1437. http://dx.doi.org/10.1110/ps.041312805

Zreiqat H, Howlett C, Zannettino A, et al., 2002, Mechanisms of magnesium-stimulated adhesion of osteoblastic cells to commonly used orthopaedic implants. J Biomed Mater Res A, 62(2): 175–184. http://dx.doi.org/10.1002/jbm.10270

Bouvard D, Pouwels J, De Franceschi N, et al., 2013, Integrin inactivators: Balancing cellular functions in vitro and in vivo. Nat Rev Mol Cell Biol, 14(7): 430–442. http://dx.doi.org/10.1038/nrm3599

Bourboulia D and Stetler-Stevenson W G, 2010, Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs): Positive and negative regulators in tumor cell adhesion. Semin Cancer Biol, 20(3): 161–168. http://dx.doi.org/10.1016/j.semcancer.2010.05.002

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2017-11-01

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Vol. 4 No. 1 (2018): International Journal of Bioprinting

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