Protocol to isolate and culture primary mouse feto-placental endothelial cells.
Accepted version
Peer-reviewed
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Repository DOI
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Authors
Sandovici, Ionel https://orcid.org/0000-0001-5674-4269
Reiterer, Moritz
Constância, Miguel
Branco, Cristina M
Abstract
In the mouse, feto-placental endothelial cells (FPEC) line the inner surface of the feto-placental blood vessels located within placental labyrinthine zone and play critical roles in placental development and function. Here, we present a detailed protocol for isolation and culture of primary mouse FPEC, as well as two complementary methods (immunohistochemistry staining and flow cytometry analysis) to assess their purity. These cells are suitable for downstream ex vivo studies to investigate their functional properties, both in normal and pathological contexts. For complete details on the use and execution of this protocol, please refer to Sandovici et al. (2022).
Description
Keywords
Cell biology, Cell culture, Cell isolation, Developmental biology, Flow cytometry/Mass cytometry, Microscopy, Tissue engineering, Female, Pregnancy, Animals, Mice, Endothelial Cells, Placenta, Flow Cytometry
Journal Title
STAR Protoc
Conference Name
Journal ISSN
2666-1667
2666-1667
2666-1667
Volume Title
Publisher
Elsevier BV
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Sponsorship
Medical Research Council (MC_UU_12012/4)
Biotechnology and Biological Sciences Research Council (BB/H003312/1)
MRC (MC_UU_00014/4)
Medical Research Council (MC_PC_12012)
Biotechnology and Biological Sciences Research Council (BB/H003312/1)
MRC (MC_UU_00014/4)
Medical Research Council (MC_PC_12012)
This work was supported by the Biotechnology and Biological Sciences Research Council (grant BB/H003312/1 to M.C.) and the Medical Research Council (MRC_MC_UU_12012/4 to M.C.).