ISHS Acta Horticulturae 586: IV International Symposium on Olive Growing
TWO NECROVIRUS ISOLATES WITH PROPERTIES OF OLIVE LATENT VIRUS 1 AND OF TOBACCO NECROSIS VIRUS FROM OLIVE IN PORTUGAL |
| Authors: | M.R.F. Félix, M.I.E. Clara |
| Keywords: | Olea europaea, viruses, Olive latent virus 1, Tobacco necrosis virus. |
| DOI: | 10.17660/ActaHortic.2002.586.155 |
| Abstract:
Two distinct virus isolates were purified from Nicotiana benthamiana plants previously inoculated with extracts of fruits from olive cultivars Galega and Verdeal Alentejana grown in Portugal. Both Gl and VA isolates had isometric particles c. 28 nm in diameter, sedimenting as a single component at 110 S, possessed a coat protein made up of a major polypeptide c. 30 kDa and an RNA genome consisting of a major species of 1.4 x 106 Da, Gl isolate had an additional low mol. wt RNA of 0.1 x 106 Da. Other differences resided in the symptomatology induced in N. benthamiana and in serological properties. Gl isolate strongly reacted with an antiserum to an Olive latent virus 1 (OLV-1) isolate from Italy, but VA did not. Conversely, VA strongly reacted with Tobacco necrosis virus (TNV) antisera, whereas Gl isolate and OLV-1 did not. These results clearly identify Gl and VA as isolates of OLV-1 and TNV, respectively. | |
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