Vet Med - Czech, 2015, 60(11):654-661 | DOI: 10.17221/8534-VETMED

Reduction of Chlamydophila-felis-associated signs by roxithromycin treatment regimen in cats showing doxycycline intoleranceOriginal Paper

K. Ploneczka-Janeczko, J. Bania, K. Bierowiec, M. Kielbowicz, Z. Kielbowicz
Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland

Chlamydophila felis (C. felis) causes chronic conjunctivitis in cats, and is frequently treated with tetracyclines. However, tetracyclines may cause gastrointestinal side effects, such as vomiting, loss of appetite, diarrhoea, and increased liver enzyme activity in some pets. We evaluated the effect of a four-week treatment regimen with roxithromycin - RXM (Rulid® Sanofi-Aventis, France) in 14 cats with conjunctivitis that tested C. felis-positive, and exhibited doxycycline intolerance. The treatment was given for four weeks. Assessment of clinical disease was performed on Day 0 and Day 56. Changes in severity of clinical signs were assessed on a three-step scale (increased, decreased, or no changes) every week of the therapy (Days 7, 14, 21, and 28). Additionally, conjunctival swabs were collected on Days 0 and 56 from each animal, and the C. felis-count was assessed through amplification of the ompA gene by real-time PCR with fluorogenic probes and normalisation to the feline DNA signal. Animals receiving RXM improved regarding the ophtalmological parameters related to manifestation of conjunctivitis, severity of ocular discharge and nasal discharge and breathing patterns (P ≤ 0.05). A visible improvement in relation to these parameters was already observed after two weeks of RXM administration. C. felis-counts decreased in nine cats that were given RXM, but in the other five C. felis was not eliminated. Interestingly, however, aggravation of symptoms was not observed in these five animals. Further studies are needed to fully confirm that a reduction of clinical signs and pathogen counts under conditions of natural infection can be attributed to RXM treatment, since there was no control group that received placebo or another drug in this study. The present results also indicate that in some cases 28 days of RXM administration will not be sufficient to eliminate infection.

Keywords: cats; chlamydophilosis; conjunctivitis; alternative therapy; roxythromycin RXM

Published: November 30, 2015  Show citation

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Ploneczka-Janeczko K, Bania J, Bierowiec K, Kielbowicz M, Kielbowicz Z. Reduction of Chlamydophila-felis-associated signs by roxithromycin treatment regimen in cats showing doxycycline intolerance. Vet Med-Czech. 2015;60(11):654-661. doi: 10.17221/8534-VETMED.
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    References

    1. Amsden GW (2005): Anti-inflammatory effects of macrolides-an underappreciated benefit in the treatment of community acquired respiratory tract infection and chronic inflammatory pulmonary conditions? Journal of Antimicrobial Chemotherapy 55, 10-21. Go to original source... Go to PubMed...
    2. Beshir KB, Hallet LR, Ezieula AC, Bailey R, Watson J,Wright SG, Chiodini P, Polley SD, Sutherland CJ (2010): Measuring the efficacy of anti-malarial drugs in vivo: quantitative PCR measurement of parasite clearance. Malaria Journal 9, 312. doi:10.1186/1475-2875-9-312. Go to original source... Go to PubMed...
    3. Chalker VJ, Owen WM, Paterson CJ, Brownlie J (2004): Development of a polymerase chain reaction for the detection of Mycoplasma felis in domestic cats. Veterinary Microbiology 100, 77-82. Go to original source... Go to PubMed...
    4. Dean R, Harley R, Helps C, Canney S, Gruffydd-Jones TJ (2005): Use of quantitative real-time PCR to monitor the response of Chlamydophila felis infection to doxycycline treatment. Journal of Clinical Microbiology 43, 1858-1864. Go to original source... Go to PubMed...
    5. Gerhard N, Schultz BS, Werckhentin C, Hartmann K (2006): Pharmacokinetics of enrofloxacin and its efficacy in comparison with doxycycline in the treatment of Chlamydophila felis infection in cats with conjunctivitis. Veterinary Record 159, 591-594. Go to original source... Go to PubMed...
    6. German AJ, Cannon MJ, Dye C, Booth MJ, Pearson GR, Reay CA, Gruffydd-Jones TJ (2005): Oesophageal strictures in cats associated with doxycycline therapy. Journal of Feline Medicine and Surgery 7, 33-41. Go to original source... Go to PubMed...
    7. Hartmann AD, Helps CD, Lappin MR, Werckhentin C, Hartmann K (2008): Efficacy of pradofloxacin in cats with feline upper respiratory tract disease due to Chlamydophila felis or Mycoplasma infections. Journal of Veterinary Internal Medicine 22, 44-52. Go to original source... Go to PubMed...
    8. Hartmann AD, Hawley J, Werckhentin C, Lappin MR, Hartmann K (2010): Detection of bacterial and viral organisms from the conjunctiva of cats with conjunctivitis and upper respiratory tract disease. Journal of Feline Medicine and Surgery 12, 785-782. Go to original source... Go to PubMed...
    9. Helps CD, Reeves N, Egan K, Howard P, Harbour D (2003): Detection of Chlamydophila felis and feline herpesvirus by multiplex real-time PCR analysis. Journal of Clinical Microbiology 41, 2734-3736. Go to original source... Go to PubMed...
    10. Koopaei MN, Maqhazei MS, Mostavi SH, Jamalifar H, Samadi N, Amini M, Malek SJ, Darvishi B, Atyabi F, Dinarvand R (2012): Enhanced antibacterial activity of roxithromycin loaded pegilated poli-lactide-co-glycolide nanoparticles. Daru 20, 92.doi: 10.1186/2008-2231-20-92. Go to original source... Go to PubMed...
    11. Krayenbuehl PA, Wiesli P, Maly FE, Vetter W, Schultess G (2005): Progression of peripheral arterial occlusive disease is associated with Chlamydia pneumoniae seropositivity and can be inhibited by antibiotic treatment. Atherosclerosis 179, 103-110. Go to original source... Go to PubMed...
    12. Livak KJ, Schmidtgen TD (2001): Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. Methods 25, 402-408. Go to original source... Go to PubMed...
    13. Marsilio F, Di Martino B, Di Francesco C (2004): Use of a duplex-PCR assay to screen for feline herpesvirus-1 and Chlamydophila spp. in mucosal swabs from cats. New Microbiologica 27, 287-292. Go to original source... Go to PubMed...
    14. Nakano T, Ohashi Y, Tanaka A, Kakinoki Y, Washio Y, Nakai Y (1998): Roxythromycin reinforces epithelial defence function in rabbit trachea. Acta Oto-Laryngologica 538 (Suppl.), 233-238. Go to original source... Go to PubMed...
    15. Owen WM, Sturgess CP, Harbour DA, Egan K, GruffyddJones TJ (2003): Efficacy of azithromycin for the treatment of feline chlamydophilosis. Journal of Feline Medicine and Surgery 5, 305-311. Go to original source... Go to PubMed...
    16. Padovan J, Ralic J, Letvus V, Milic A, Benetic Mihajlevic V (2012): Investigating the barriers to bioavailability of macrolide antibiotics in the rat. European Journal of Drug Metabolism and Pharmacokinetics 37, 163-171. Go to original source... Go to PubMed...
    17. Preac-Mursic V, Vilske B, Schierz G, Suss E, Gross B (1989): Comparative antimicrobial activity of the new macrolides against Borrelia burgdorferi. European Journal of Clinical Microbiology and Infectious Diseases 8, 651-653. Go to original source... Go to PubMed...
    18. Sandoz KM, Rockey DD (2010): Antibiotic resistance in Chlamydiae. Future Microbiology 5, 1427-1442. Go to original source... Go to PubMed...
    19. Schulz BS, Hupfauer S, Ammer H, Sauter-Louis C, Hartmann K (2011): Suspected side effects of doxycycline use in dogs - a retrospective study of 386 cases. Veterinary Record 169, 229. doi:10.1136/vr.d4344. Go to original source... Go to PubMed...
    20. Schulz BS, Zauscher S, Ammer H, Sauter-Louis C, Hartmann K (2013): Side effects suspected to be related to doxycycline treatment. Veterinary Record 172, 184. doi:10.1136/vr.101031. Go to original source... Go to PubMed...
    21. Steel HC, Theron AJ, Cockeran R, Anderson R, Feldman C (2012): Pathogen- and host-directed anti-inflammatory activities of macrolide antibiotics. Mediators of Inflammation 2012, 2012: 58426. doi: 10.1155/2012/584262. Go to original source... Go to PubMed...
    22. Stephens RS, Meyers G, Eppinger M, Bavoil PM (2009): Divergence without difference: phylogenetics and taxonomy of Chlamydia resolved. FEMS Immunology and Medical Microbiology 55, 115-119. Go to original source... Go to PubMed...
    23. Sturgess CP, Gruffydd-Jones TJ, Harbour DA, Jones LR (2001): Controlled study of the efficacy of clavulanic acidpotentiated amoxycillin in the treatment of Chlamydia psittacii in cats. Veterinary Record 149, 73-76. Go to original source... Go to PubMed...
    24. Sugiura Y, Ohashi Y, Nakai Y (1997a): Roxythromycin stimulates the mucociliary activity of the Eustachian Tube and modulates neuthrophil activity in the healthy guinea pigs. Acta Oto-Laryngologica 531 (Suppl.), 34-38. Go to original source... Go to PubMed...
    25. Sugiura Y, Ohashi Y, Nakai Y (1997b): Roxythromycin prevents endotoxin-induced otitis media with effusion in guinea pigs. Acta Oto-Laryngologica 531 (Suppl.), 39-51. Go to original source... Go to PubMed...
    26. Sykes JE (2005): Feline Chlamydophilosis. Clinical Techniques in Small Animal Practice 20, 129-134. Go to original source... Go to PubMed...
    27. Tamaoki J, Kadota J, Takizawa H (2004): Clinical implication of the immunomodulatory effects of macrolides. The American Journal of Medicine 117 (Suppl. 9A), 5-11. doi: 10.1016/j.amjmed.2004.07.023 Go to original source... Go to PubMed...
    28. Worm AM, Hoff G, Kroon S, Petersen CS, Christensen JJ (1989): Roxithromycin compared with erythromycin against genitourinary chlamydial infections. Genitourinary Medicine 65, 35-38. Go to original source... Go to PubMed...
    29. Wyrick PB, Knight ST (2004): Pre-exposure of infected human endomethrial epithelial cells to penicillin in vitro renders Chlamydia trachomatis refractory to azithromycin. Journal of Antimicrobial Chemotherapy 54, 79-85. Go to original source... Go to PubMed...

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