J. For. Sci., 2007, 53(2):88-92 | DOI: 10.17221/2138-JFS

The use of silica sand in micropropagation of woods

H. Prknová
Faculty of Forestry and Environment, Czech University of Life Sciences in Prague, Prague, Czech Republic

Cultures in vitro made in agar are rather precarious, because gel strength varies both with the medium formula used and the source and grade of agar. Any solidifying agent (like for example agar) should be strong enough to support cultivated plantlets, yet liquid enough to allow the nutrients and drossy products from plants through the medium. It should also be a chemically inert material. Agar, especially in acid solutions, is an undefined constituent of culture media, namely in the mentioned properties. Silica sand, used in cultures of herbs up to the present time, is applicable also in cultures of Sorbus sudetica. The required acid medium is exactly defined if sand is substituted for agar. Similar cultures of wood species, including conifers, will be realized in future research.

Keywords: micropropagation; agar; silica sand; acid medium; Sorbus sudetica

Published: February 28, 2007  Show citation

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Prknová H. The use of silica sand in micropropagation of woods. J. For. Sci.. 2007;53(2):88-92. doi: 10.17221/2138-JFS.
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