Czech J. Food Sci., 2023, 41(4):287-294 | DOI: 10.17221/114/2022-CJFS

Development of a novel invA gene-based real-time PCR assay for the detection of Salmonella in foodOriginal Paper

Marko Dmitric1*, Dejan Vidanovic1, Kazimir Matovic1, Bojana Tesovic1, Milanko Sekler1, Ivan Vicic2, Nedjeljko Karabasil2
1 Veterinary Specialised Institute Kraljevo, Kraljevo, Serbia
2 Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia

In this study, the primers and the probe were designed, and a completely new real-time polymerase chain reaction (PCR) protocol for detecting the invA gene of Salmonella in food was optimised and validated in-house. The inclusivity test used 76 different Salmonella isolates with no false-negative results. The exclusivity was tested using 45 non-Salmonella microorganisms with no false-positive results. The method was also successfully applied while examining five different artificially contaminated food categories. The results were compared to the standard method (ISO 6579-1) and two previously validated real-time PCR methods. The developed assay is sensitive and specific for rapidly detecting Salmonella in food.

Keywords: molecular detection; food safety; validation; pathogen; artificial contamination

Accepted: August 8, 2023; Prepublished online: August 17, 2023; Published: August 29, 2023  Show citation

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Dmitric M, Vidanovic D, Matovic K, Tesovic B, Sekler M, Vicic I, Karabasil N. Development of a novel invA gene-based real-time PCR assay for the detection of Salmonella in food. Czech J. Food Sci.. 2023;41(4):287-294. doi: 10.17221/114/2022-CJFS.
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