Deneysel Kriptorşidizmde Elektron Mikroskopik İnceleme ve Mast Hücre-Fibrozis İlişkisi
Öz
Öz.
Amaç: Bu çalışmada deneysel tek taraflı kriptorşidizmli sıçanlarda elektron mikroskobik inceleme ve testiküler mast hücreleri ve fibrozis arasındaki ilişkinin araştırılması amaçlanmıştır
Gereç ve Yöntemler: Çalışmamızda 15-17 günlük 10 tane erkek Sprague Dawley cinsi sıçan kontrol ve kriptorşid olarak iki gruba ayrıldı. Deney hayvanlarının kriptorşidizm modeli oluşturulduktan 15 gün sonra testisleri alındı. Bu dokulara ışık ve elektron mikroskobik incelemeler için rutin takip prosedürleri uygulandı. Işık mikroskopik incelemeler için Hematosilen- Eozin (H-E) ve Masson’s Trichrom (TRI) boyaması yapıldı. Ayrıca immün reaktivite çalışmaları için Anti Mast Cell Tryptase antikoru kullanarak mast hücre sayısı değerlendirildi.
Bulgular: Çalışmamızda kriptorşidik testislerde insterstisyel alanda seminifer tübüllerin birbirinden uzaklaşması, germ hücrelerin bazal membrandan ayrılması, perivasküler fibrozis, ödem, konjesyon, hemoraji, interstisyel alanda ve peritübüler alanda fibrozisin artışı, seminifer tübül çaplarında ise azalma gözlemlendi. Elektron mikroskopide seminifer tübül ve insterstisyumda şiddetli dejeneratif değişiklikler, bazal lamina kalınlığının belirgin şekilde arttığı, Sertoli hücrelerinin sitoplazmalarında yoğun düz endoplazmik retikulum vakuolizasyonu ve anormal kristaya sahip mitokondriyonlar, Sertoli-Sertoli hücreleri ve Sertoli-spermatojenik hücreleri arasındaki sıkı bağlantılar ile spermatojenik hücrelerin kendi aralarındaki sitoplazmik köprüler bütünlüğünü yitirmişti. Peritübüler alandaki fibrozis ile mast hücre sayısı arasında pozitif bir korelasyon saptandı.
Sonuç:.Çalışmamızdaki deneysel kriptorşidizm modelinde mast hücre sayısı ile fibrozis arasında pozitif yönde bir korelasyon olduğu tesbit edildi.
Anahtar Kelimeler: Kriptorşidizm; Mast hücresi; Fibrozis; Elektron mikroskobik yapı.
Electron Microscopic Examination and The Relationship Between Mast Cell-Fibrosis in Experimental Cryptorchidism
Abstract
Purpose: In this study, it was aimed to investigate the relationship between electron microscopic examination and testicular mast cells and fibrosis in rats with experimental unilateral cryptorchidism.
Materials and Methods: In this study, 15-17 day old 10 male Sprague Dawley rats were divided into two groups as control group and experimental group with cryptorchidism. The testes of the rats were extracted fifteen days after the Cryptorchidism model had been developed in rats. These tissues were processed according to standard methods for the light and electron microscopic analysis. Masson’s trichrome (TRI) and Hematoxylin-eosin (H-E) stains were used for light microscopic examinations. Also, the mast cells were evaluated by using Anti Mast Cell Tryptase anticors for immuneractivity studies.
Results: In our study, the diverge of seminiferous tubules from each other in interstitial space, separation/ disconnection of germ cells from basal membrane, perivascular fibrosis, edema,congestion, hemorrhage, the increament in the fibrosis in inertitial space and peritubullar space, and the decreament in the diameters of seminiferous tubules were observed in testes with cryptorchidism. Electron microscopic analysis revealed severe degenerative changes in seminiferous tubules and interstisium, significant increase in the thickness of basal lamina, intense smooth endoplasmic reticulum vacuolization and mitochodrions with anormal crista in the cytoplasm of sertoli cells, destruction in tight junctions between the sertoli cells each other and also sertoli cells and spermotogenic cells, decomposition of cytoplasmic bridges between the spermatogenic cells. The possitive correlation was detected between the number of the mast cells and fibrosis in peritubuller space.
Conclusion: The positive correlation was detected between the number of the mast cells and fibrosis in experimental cryptorchidism model.
Keywords: Cryptorchidism; Mast cell; Fibrosis; Electron microscopic structure.
Anahtar Kelimeler
Kriptorşidizm; Mast hücresi; Fibrozis; Elektron mikroskobik yapı
Destekleyen Kurum
Gaziantep Üniversitesi Bilimsel Araştırma Projeleri Yönetim Birimi (BAPYB)
Proje Numarası
BAP No: TF.YLT.18.12
Kaynakça
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