With experimentally dehydrated dogs, general conditions of neursecretory cells of supraoptic and paraventricular nuclei and changes in the shapes and forms, arrangements, and distributions etc. of mitochondria, GOLGIapparatus, NISSLsubstance, and neurosecretory material were observed.
1. The size of a cell nucleus is enlarged in the dehydrated condition, manifesting hyperactivity of cell function.
2. Intranucleolar vesicles are increased markedly, and taking the irregularization of the arrangement and distribution of NISSL-substance also into account, a change of the mechanism of RNA metabolism is suggested.
3. Mitochondria are observed to grow long rod-like or filamentous and, in addition, to tend to collect themselves in the cytoplasm nearer to the nucleus. It is considered that mitochondria would probably participate in the formation of secretory material indirectly through their enzyme system.
4. GOLGI-apparatus, in the dehydrated condition, are developed remarkably, displaying frequently a complicated network formation. On the other hand, many cells with GOLGI-apparatus of fragmented form are observed. These findings are considered to be symptoms of hyperactivities of cell-functions.
5. Individual NISSL bodies become irregular in shape and form, unequal in size, and confused in arrangement, and massive NISSL-substances appear frequently in the central area of a cell, esp. adhering to the nuclear membrane. These findings are considered to signify a condition where a good many material to be consumed for the production of the secretory granule are being supplied answering to a markedly increasing demand of adiuretin. The condition might be accompanied by the change of the mechanism of RNA metabolism.
6. Of secretory material in the cell, in the dehydrated condition, an extremely small amount of them in fine granular form are only observed scattering in the cytoplasm near the nucleus. This might signify that the secretory material, answering to an increasing demand, is quickly transported to the posterior lobe, with little time to stay in the cell body.
7. Such cytological findings on neurosecretory cells in the dehydrated condition as are described above are considered to show that the production of the secretory material accompanied by some changes, more or less, of the mechanism of RNA metabolism, is in a highly elevated condition.