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Pathogen Identification of Anthracnose Disease on Coffea arabica in Guangdong Province and Screening of FungicidesChinese Full Text

XU Dandan;SHI Liyun;ZHANG Yu;LIN Zemian;JIANG Zide;QIAO Fang;Department of Applied Chemistry and Biotechnology/Postdoctoral Innovation Practice Base,Shenzhen Polytechnic;College of Plant Protection, South China Agricultural University/Guangdong Key Laboratory of Microbial Signals and Disease Control;

Abstract: 【Objective】The study was conducted to clarify the pathogen causing anthracnose disease of Coffea arabica in Guagdong Province,and to screen out control fungicides.【Method】Tissue isolation method was used to isolate and purify the pathogen,and pathogenicity test was conducted by using Koch’s postulates.The pathogen was identified based on morphological characteristics and analysis of phylogenetic sequences(ITS,TUB2,GAPDH,CHS,ACT and GS).Moreover,the inhibitory effects of four common fungicides on the pathogen were measured according to mycelial growth.【Result】The obtained strains were identified as Colletotrichum fructicola,C.siamense and C.musicola.The results of pathogenicity assay indicated that all the selected isolates could infect leaves.In vitro test on two highly pathogenic strains (C. fructicola CA-13 and C. siamense CA-16)showed that prochloraz,pyraclostrobin and thiophanate-methyl strongly inhibited mycelium growth and their EC50 values were under 0.1 mg/L.【Conclusion】The dominant pathogen causing anthracnose disease on C. arabica leaves were C. fructicola and C. siamense;prochloraz,pyraclostrobin and thiophanate-methyl could be used to control this disease as the first choice.
  • DOI:

    10.16768/j.issn.1004-874X.2021.02.013

  • Series:

    (D) Agriculture

  • Subject:

    Plant Protection

  • Classification Code:

    S435.712

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