概要
前哨淋巴结绘图和肿瘤边界的确定能有效减少手术干预及提高患者的无复发生存率,在癌症手术中发挥重要作用。自体荧光成像(AFI)技术可提高肿瘤检测率且优化手术干预范围,可惜目前尚无可用于混合成像的荧光标记药物,而且荧光染料与叶酸结合时易出现光漂白现象。本研究首次报道了一种可用于同步混合成像的叶酸纳米传感器,其光稳定性优异且与AFI内窥镜兼容。经过量子点表面功能化处理后,该纳米传感器能够与MCF-7癌细胞有效结合,从而可使用普通AFI设备进行同步混合成像诊断,进行前哨淋巴结绘图和确定肿瘤边界,这不仅降低诊断的成本,也提高了诊断的可靠性。
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Acknowledgments
We thank Roman IVANOV, Vice-President for Development and Research of CJSC BIOCAD (Saint Petersburg, Russia), for the opportunity to obtain flow cytofluorometry and in vivo imaging data. We are also very grateful to Alexey GRACHEV, the Head of the Laboratory of Stromal Tumor Cell Biology at the N. N. Blokhin Cancer Research Centre (Moscow, Russia), for assistance in obtaining cytotoxicity data, and to Kirill CHEREDNICHENKO, a senior researcher from Gubkin Russian State University of Oil and Gas (NRU) (Moscow, Russia), for providing transmission electron microscopy (TEM) data.
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Denis KUZNETSOV: Conceptualization, Methodology, Data curation, Formal analysis, Project administration, Writing original draft, Visualization. Sergey DEZHUROV and Dmitri KRYLSKY: Conceptualization, Data curation, Methodology, Formal analysis, Writing review & editing. Valery NOVIKOV and Valery NESCHISLIAEV: Writing review & editing, Formal analysis. Anastasiia KUZNETSOVA: Validation, Data curation, Visualization.
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Denis KUZNETSOV, Sergey DEZHUROV, Dmitri KRYLSKY, Valery NOVIKOV, Valery NESCHISLIAEV, and Anastasiia KUZNETSOVA declare that they have no conflict of interest.
All institutional and national guidelines for the care and use of laboratory animals were followed.
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Kuznetsov, D., Dezhurov, S., Krylsky, D. et al. Use of folic acid nanosensors with excellent photostability for hybrid imaging. J. Zhejiang Univ. Sci. B 23, 784–790 (2022). https://doi.org/10.1631/jzus.B2200107
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DOI: https://doi.org/10.1631/jzus.B2200107