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Alternaria toxin-induced resistance against rose aphids and olfactory response of aphids to toxin-induced volatiles of rose plants

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Abstract

The search for active toxins for managing weeds or plant diseases is believed to be a promising avenue of investigation. However, the effects of Alternaria toxins on insects have just begun to be investigated. Bioactivities of toxins from four strains of Alternaria alternata on Rosa chinensis and rose aphid Macrosiphum rosivorum were tested in the present study. At a concentration of 50.0 μg/ml, the crude extract (toxin) of strain 7484 was found not to be harmful to rose plants with excised leaf-puncture method (P≥0.079), and rose plants showed enhanced resistance to rose aphids when this Alternaria toxin was sprayed on the plants (P≤0.001). However, this toxin caused no detrimental effects on aphids in insecticidal bioassay at a concentration of 10.0 to 160.0 μg/ml (P≥0.096). Therefore, the Alternaria toxin had significantly induced the resistance of rose plants against rose aphids, demonstrating that the resistance mechanism triggered by the Alternaria toxin in the rose plant may also be used by the plant to defend itself against insects. Further bioassays aimed to discover the olfactory responses of aphids to the toxin-induced volatiles of host plants. The aphids were significantly more attracted to both volatiles emitted and collected from control rose plants than to both volatiles emitted and collected from the toxin-treated rose plants (P≤0.014). This result showed that the toxin-induced resistance related to the volatile changes of host plants.

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Authors and Affiliations

  1. Key Laboratory of Forest Disaster Warning and Control in Yunnan Province, Southwest Forestry University, Kunming, 650224, China

    Fa-zhong Yang, Li Li & Bin Yang

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  1. Fa-zhong Yang
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  2. Li Li
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  3. Bin Yang
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Correspondence to Bin Yang.

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Project supported by the Scientific and Technological Department (No. 2008CD140) and the Education Department (No. 08z0027) of Yunnan Province in China

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Yang, Fz., Li, L. & Yang, B. Alternaria toxin-induced resistance against rose aphids and olfactory response of aphids to toxin-induced volatiles of rose plants. J. Zhejiang Univ. Sci. B 13, 126–135 (2012). https://doi.org/10.1631/jzus.B1100087

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  • DOI: https://doi.org/10.1631/jzus.B1100087

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