The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
Electrophoretic Separation of X- and Y-Chromo-some-Bearing Sperm in Human Semen
MASAFUMI SHIRAISHOTARO MATSUDASHIRO MITSUKAWA
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JOURNAL FREE ACCESS

1974 Volume 113 Issue 3 Pages 273-281

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Abstract

In an attempt to galvanically separate X- and Y-bearing sperm in human semen while maintaining it in as much a natural state as possible, we performed a series of experiments and have identified D. C., 2.3 V, 200μA, 30 minutes as the most adequate means of achieving this, with negligible interference with sperm motility, the balance of seminal electrolytes or pH. The seminal galvanization processed in this condition induced X-bearing sperm to the cathode and Y-bearing sperm to the anode, suggesting the possibility that this separately attracted semen could be used for the artificial insemination with the purpose of obtaining desired sexes in offspring. It is advised, however, that any practice with this method be limited to the field of livestock rearing.

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© Tohoku University Medical Press
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