International Journal of Medicinal Mushrooms

ISSN for PRINT: 1521-9437

For Online Access

Best Paper Award Selection - Editorial Board Site

2008, Volume10

Issue 2

97 pages

DOI: 10.1615/IntJMedMushr.v10.i2

Issue price - $161.00

Yew Keong Choong
Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti of Putra Malaysia, 43400 UPM, Selangor, Malaysia

Mohamed Mustapha Noordin
Department of Pathology and Veterinary Microbiology, Faculty of Veterinary Medicine, Universiti of Putra Malaysia, 43400 UPM, Selangor, Malaysia

Suhaila Mohamed
Department of Food Science and Management, Faculty of Food Science and Technology, Universiti of Putra Malaysia, 43400 UPM, Selangor, Malaysia

Abdul Manaf Ali
Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 UPM, Selangor, Malaysia

Nor Aini B. Umar
Department of Pathology, Hospital Universiti Kebangsaan Malaysia, UKM, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Malaysia

Chow Chin Tong
Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti of Putra Malaysia, 43400 UPM, Selangor, Malaysia

The cytotoxic effect of crude extracts of three Ganoderma species (G. lucidum, G. tropicum, and G. tsugae) was screened against the human estrogen-independent cell line, MDA-MB-435 using a microculture tetrazolium (MTT) assay. The crude extract obtained from the fruiting body of Ganoderma lucidum by hot water extraction procedures had IC₅₀ values ranging from 140 to 350 μg mL⁻¹. Acridine-orange/propidium iodine staining, electron microscopy, and transmission electron microscopy showed that the crude extract caused both apoptosis and necrosis. The G. lucidum mycelial crude extract was most effective, with an IC₅₀ value of 148 μg mL⁻¹.

DOI: 10.1615/IntJMedMushr.v10.i2.20 Download article, 115-125 pages

Article price - $35.00

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