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Detection of Sourgrass (Digitaria insularis) Biotypes Resistant to Glyphosate in Brazil

Published online by Cambridge University Press:  20 January 2017

Leonardo Bianco de Carvalho ,
Hugo Cruz-Hipolito ,
Fidel González-Torralva ,
Pedro Luis da Costa Aguiar Alves ,
Pedro Jacob Christoffoleti  and
Rafael De Prado
Leonardo Bianco de Carvalho*
Affiliation:
Department of Applied Biology to Agropecuary, São Paulo State University, Brazil 14884-900
Hugo Cruz-Hipolito
Affiliation:
Department of Agricultural Chemistry and Edaphology, University of Córdoba, Spain 14071
Fidel González-Torralva
Affiliation:
Department of Agricultural Chemistry and Edaphology, University of Córdoba, Spain 14071
Pedro Luis da Costa Aguiar Alves
Affiliation:
Department of Applied Biology to Agropecuary, São Paulo State University, Brazil 14884-900
Pedro Jacob Christoffoleti
Affiliation:
Department of Crop Science, University of São Paulo, Brazil 13418-900
Rafael De Prado
Affiliation:
Department of Agricultural Chemistry and Edaphology, University of Córdoba, Spain 14071
*
Corresponding author's E-mail: agrolbcarvalho@gmail.com
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Abstract

Sourgrass is a perennial weed infesting annual and perennial crops in Brazil. Three biotypes (R1, R2, and R3) of sourgrass suspected to be glyphosate-resistant (R) and another one (S) from a natural area without glyphosate application, in Brazil, were tested for resistance to glyphosate based on screening, dose-response, and shikimic acid assays. Both screening and dose-response assays confirmed glyphosate resistance in the three sourgrass biotypes. Dose-response assay indicated a resistance factor of 2.3 for biotype R1 and 3.9 for biotypes R2 and R3. The hypothesis of a glyphosate resistance was corroborated on the basis of shikimic acid accumulation, where the S biotype accumulated 3.3, 5.0, and 5.7 times more shikimic acid than biotypes R1, R2, and R3, respectively, 168 h after treatment with 157.50 g ae ha−1 of glyphosate. There were no differences in contact angle of spray droplets on leaves and spray retention, indicating that differential capture of herbicide by leaves was not responsible for resistance in these biotypes. The results confirmed resistance of sourgrass to glyphosate in Brazil.

Keywords

Glyphosatesourgrass, Digitaria insularis (L.) Mez ex Ekman, TRCINHerbicide resistanceshikimic acidspray retentioncontact angle
Type
Weed Biology and Ecology
Information
Weed Science , Volume 59 , Issue 2 , June 2011 , pp. 171 - 176
Copyright
Copyright © Weed Science Society of America 

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