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Morphological Changes of Isolated Osteoclasts in Cell Culture Due to Different Biomaterials

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Abstract

Osteoclasts have been isolated in primary cell culture using femoral bone of laying hens being fed on an eight day calcium free diet. Placing these cells on the surface of fixed cortico-femoral chicken bone provoked the feature of resorption pits proving that they are able to resorb bone.

After placing osteoclasts on different biomaterials (Aluminumoxide ceramics, teflon, carbon fibre reinforced polysulphone, polymethylmethacrylate, polydioxanone) scanning electron microscopy was performed. Different materials provoke different morphological features of these cells, probably due to functional variations as a response to the changing surfaces. Adhesion was feasible on all the surfaces, uptake of small surface particles was possible and cell fusion took place on most materials suggesting acceptance of the tested biomaterials by the cells.

The results show that morphological changes of isolated osteoclasts in cell culture can be detected due to different functional challenges of the surfaces of different biomaterials.

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Authors and Affiliations

  1. Department of Oral and Maxillo-Facial Surgery, Christian-Albrechts-University Kiel, Germany

    J. Thomas Lambrecht, R. Ewers, A. Kerscher & R. Jentzsch

Authors
  1. J. Thomas Lambrecht
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  2. R. Ewers
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  3. A. Kerscher
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  4. R. Jentzsch
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Lambrecht, J.T., Ewers, R., Kerscher, A. et al. Morphological Changes of Isolated Osteoclasts in Cell Culture Due to Different Biomaterials. MRS Online Proceedings Library 110, 317–323 (1987). https://doi.org/10.1557/PROC-110-317

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  • DOI: https://doi.org/10.1557/PROC-110-317

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