Abstract
Magnetic nanoparticle-vesicle aggregates (MNPVs), a controlled release nanostructure, have been enhanced with the inclusion of a novel galactose terminated lipid for cell targeting. Quartz crystal microgravimetry with dissipation (QCM-D) demonstrated that the galactose headgroup was available to bind Erythrina Crista-galli lectin (ECL) when the lipid was incorporated into a lipid bilayer. Similarly, UV-visible spectrophotometry indicated that ECL recognized the galactose headgroup in vesicles, leading to vesicle adhesion and aggregation. Finally, confocal fluorescence microscopy was used to assess the galactose-mediated interaction of both vesicles and MNPVs with HepG2 human hepatocellular carcinoma cells expressing the asialoglycoprotein (ASGPR) galactose receptor.
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Acknowledgments
The authors would like to thank Dr. Louise Carney for assistance with cell culture and fluorescence microscopy techniques, Dr. Usha Devi and Biolin Scientific UK for providing use of a QCM-D instrument. The BBSRC is also thanked for providing financial support.
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Booth, A., Coxon, T.P., Gough, J.E. et al. Targeting of a magnetic bionanomaterial to HepG2 human hepatocellular carcinoma cells using a galactose terminated lipid. MRS Online Proceedings Library 1688, 12–17 (2014). https://doi.org/10.1557/opl.2014.517
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DOI: https://doi.org/10.1557/opl.2014.517