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Shape and Biomechanical Characteristics of Human Red Blood Cells in Health and Disease

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Abstract

The biconcave shape and corresponding deformability of the human red blood cell (RBC) is an essential feature of its biological function. This feature of RBCs can be critically affected by genetic or acquired pathological conditions. In this review, we highlight new dynamic in vitro assays that explore various hereditary blood disorders and parasitic infectious diseases that cause disruption of RBC morphology and mechanics. In particular, recent advances in high-throughput microfluidic devices make it possible to sort/identify healthy and pathological human RBCs with different mechanobiological characteristics.

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References

  1. Y.C. Fung, Biomechanics: Mechanical Properties of Living Tissues (Springer-Verlag, New York, 1993).

    Google Scholar 

  2. W.T. Tse, S.E. Lux, Br. J. Haematol. 104, 2 (1999).

    Google Scholar 

  3. J. Li, M. Dao, C.T. Lim, S. Suresh, Biophys. J. 88, 3707 (2005).

    Google Scholar 

  4. H.W.G. Lim, M. Wortis, R. Mukhopadhyay, Proc. Nat. Acad. Sci. U.S.A. 99, 16766 (2002).

    Google Scholar 

  5. P.B. Canham, J. Theor. Biol. 26, 61 (1970).

    Google Scholar 

  6. W. Helfrich, Z. Naturforsch., C: Biosci. C 28, 693 (1973).

    Google Scholar 

  7. D.E. Discher, D.H. Boal, S.K. Boey, Phys. Rev. E 55, 4762 (1997).

    Google Scholar 

  8. D.E. Discher, D.H. Boal, S.K. Boey, Biophys. J. 75, 1584 (1998).

    Google Scholar 

  9. J. Li, G. Lykotrafitis, M. Dao, S. Suresh, Proc. Nat. Acad. Sci. U.S.A. 104, 4937 (2007).

    Google Scholar 

  10. P. Wong, J. Theor. Biol. 196, 343 (1999).

    Google Scholar 

  11. N.S. Gov, S.A. Safran, Biophys. J. 88, 1859 (2005).

    Google Scholar 

  12. Y.K. Park, C.A. Best, T. Auth, N. Gov, G. Safran, G. Popescu, S. Suresh, M.S. Feld, Proc. Nat. Acad. Sci. U.S.A. 107, 1289 (2010).

    Google Scholar 

  13. S. Eber, S.E. Lux, Semin. Hematol. 41, 118 (2004).

    Google Scholar 

  14. S. Perrotta, P.G. Gallagher, N. Mohandas, Lancet 372, 1411 (2008).

    Google Scholar 

  15. P.G. Gallagher, Curr. Hematol. Rep. 3, 85 (2004).

    Google Scholar 

  16. P.S. Becker, S.E. Lux, Clin. Haematol. 14, 15 (1985).

    Google Scholar 

  17. S.C. Liu, L.H. Derick, P. Agre, J. Palek, Blood 76, 198 (1990).

    Google Scholar 

  18. L. Da Costa, N. Mohandas, M. Sorette, M.J. Grange, G. Tchernia, T. Cynober, Blood 98, 2894 (2001).

    Google Scholar 

  19. L.D. Walensky, M. Narla, S.E. Lux, in Blood: Principles and Practice of Hematology, 2nd Edition, R.I. Handin, S.E. Lux, T.P. Stossel, Eds. (Lippincott Williams, and Wilkins, Philadelphia, 2003), p. 1709.

    Google Scholar 

  20. L. Pauling, H.A. Itano, S.J. Singer, I.C. Wells, Science 110, 543 (1949).

    Google Scholar 

  21. V.M. Ingram, Nature 178, 792 (1956).

    Google Scholar 

  22. S.H. Embury, R.P. Hebbel, M.H. Steinberg, N. Mohandas, in Sickle Cell Disease, S.H. Embury, R.P. Hebbel, N. Mohandas, M.H. Steinberg, Eds. (Raven Press, New York, 1994), p. 311.

    Google Scholar 

  23. N. Mohandas, R.P. Hebbel, in Sickle Cell Disease, S.H. Embury, R.P. Hebbel, N. Mohandas, M.H. Steinberg, Eds. (Raven Press, New York 1994), p. 205.

    Google Scholar 

  24. R.P. Hebbel, O. Yamada, C.F. Moldow, H.S. Jacob, J.G. White, J.W. Eaton, J. Clin. Invest. 65, 154 (1980).

    Google Scholar 

  25. J.M. Higgins, D.T. Eddington, S.N. Bhatia, L. Mahadevan, Proc. Nat. Acad. Sci. U.S.A. 104, 20496 (2007).

    Google Scholar 

  26. A.V.S. Hill, C.E.M. Allsopp, D. Kwiatkowski, N.M. Anstey, P. Twumasi, P.A. Rowe, S. Bennett, D. Brewster, A.J. McMichael, B.M. Greenwood, Nature 352, 595 (1991).

    Google Scholar 

  27. A. Greenwood, B. Mutabingwa, T. Malaria, Nature 415, 670 (2002).

    Google Scholar 

  28. J.P. Mills, M. Diez-Silva, D.J. Quinn, M. Dao, M.J. Lang, K.S.W. Tan, C.T. Lim, G. Milon, P.H. David, O. Mercereau-Puijalon, S. Bonnefoy, S. Suresh, Proc. Nat. Acad. Sci. U.S.A. 104, 9213 (2007).

    Google Scholar 

  29. F.K. Glenister, R.L. Coppel, A.F. Cowman, N. Mohandas, B.M. Cooke, Blood 99, 1060 (2002).

    Google Scholar 

  30. F.K. Glenister, K.M. Fernandez, L.M. Kats, E. Hanssen, N. Mohandas, R.L. Coppel, B.M. Cooke, Blood 113, 919 (2009).

    Google Scholar 

  31. H.A. Cranston, C.W. Boylan, G.L. Carroll, S.P. Sutera, J.R. Williamson, I.Y. Gluzman, D.J. Krogstad, Science 223, 400 (1984).

    Google Scholar 

  32. A.M. Dondorp, B.J. Angus, M.R. Hardeman, K.T. Chotivanich, K. Silamut, R. Ruangveerayuth, P.A. Kager, N.J. White, J. Vreeken, Am. J. Trop. Med. Hyg. 57, 507 (1997).

    Google Scholar 

  33. M. Paulitschke, G.B. Nash, J. Lab. Clin. Med. 122, 581 (1993).

    Google Scholar 

  34. S. Suresh, J. Spatz, J.P. Mills, A. Micoulet, M. Dao, C.T. Lim, M. Beil, T. Seufferlein, Acta Biomater. 1, 15 (2005).

    Google Scholar 

  35. J.P. Mills, L. Qie, M. Dao, K.S.W. Tan, C.T. Lim, S. Suresh, in Mechanical Properties of Bioinspired and Biological Materials, C. Viney, K. Katti, F.J. Ulm, C. Hellmich, Eds. (2005), 844, p. 179.

  36. Y.K. Park, M. Diez-Silva, G. Popescu, G. Lykotrafitis, W.S. Choi, M.S. Feld, S. Suresh, Proc. Nat. Acad. Sci. U.S.A. 105, 13730 (2008).

    Google Scholar 

  37. G. Bao, S. Suresh, Nat. Mater. 2, 715 (2003).

    Google Scholar 

  38. K.J. Van Vliet, J. Li, T. Zhu, S. Yip, S. Suresh, Phys. Rev. B 67, (2003).

  39. J.P. Shelby, J. White, K. Ganesan, P.K. Rathod, D.T. Chiu, Proc. Nat. Acad. Sci. U.S.A. 100, 14618 (2003).

    Google Scholar 

  40. M. Antia, T. Herricks, P.K. Rathod, PLoS Pathog. 3, 939 (2007).

    Google Scholar 

  41. T. Herricks, M. Antia, P.K. Rathod, Cell. Microbiol. 11, 1340 (2009).

    Google Scholar 

  42. S. Handayani, D.T. Chiu, E. Tjitra, J.S. Kuo, D. Lampah, E. Kenangalem, L. Renia, G. Snounou, R.N. Price, N.M. Anstey, B. Russell, Journal of Infectious Diseases 199, 445 (2009).

    Google Scholar 

  43. I. Safeukui, J.M. Correas, V. Brousse, D. Hirt, G. Deplaine, S. Mule, M. Lesurtel, N. Goasguen, A. Sauvanet, A. Couvelard, S. Kerneis, H. Khun, I. Vigan-Womas, C. Ottone, T.J. Molina, J.M. Treluyer, O. Mercereau-Puijalon, G. Milon, P.H. David, P.A. Buffet, Blood 112, 2520 (2008).

    Google Scholar 

  44. I.J. Udeinya, J.A. Schmidt, M. Aikawa, L.H. Miller, I. Green, Science 213, 555 (1981).

    Google Scholar 

  45. C.F. Ockenhouse, N.N. Tandon, C. Magowan, G.A. Jamieson, J.D. Chulay, Science 243, 1469 (1989).

    Google Scholar 

  46. D.D. Roberts, J.A. Sherwood, S.L. Spitalnik, L.J. Panton, R.J. Howard, V.M. Dixit, W.A. Frazier, L.H. Miller, V. Ginsburg, Nature 318, 64 (1985).

    Google Scholar 

  47. A.R. Berendt, D.L. Simmons, J. Tansey, C.I. Newbold, K. Marsh, Nature 341, 57 (1989).

    Google Scholar 

  48. P. Oquendo, E. Hundt, J. Lawler, B. Seed, Cell 58, 95 (1989).

    Google Scholar 

  49. D.I. Baruch, J.A. Gormley, C. Ma, R.J. Howard, B.L. Pasloske, Proc. Nat. Acad. Sci. U.S.A. 93, 3497 (1996).

    Google Scholar 

  50. J.A. Rowe, J.M. Moulds, C.I. Newbold, L.H. Miller, Nature 388, 292 (1997).

    Google Scholar 

  51. B.M. Cooke, A.R. Berendt, A.G. Craig, J. Macgregor, C.I. Newbold, G.B. Nash, Br. J. Haematol. 87, 162 (1994).

    Google Scholar 

  52. B.G. Yipp, S. Anand, T. Schollaardt, K.D. Patel, S. Looareesuwan, M. Ho, Blood 96, 2292 (2000).

    Google Scholar 

  53. C. Gray, C. McCormick, G. Turner, A. Craig, Mol. Biochem. Parasitol. 128, 187 (2003).

    Google Scholar 

  54. B.M. Cooke, F.K. Glenister, N. Mohandas, R.L. Coppel, Br. J. Haematol. 117, 203 (2002).

    Google Scholar 

  55. M. Antia, T. Herricks, P.K. Rathod, Cell. Microbiol. 10, 1968 (2008).

    Google Scholar 

  56. J.P. Brody, Y. Han, R.H. Austin, M. Bitensky, Biophys. J. 68, 2224 (1995).

    Google Scholar 

  57. J.A. Davis, D.W. Inglis, K.J. Morton, D.A. Lawrence, L.R. Huang, S.Y. Chou, J.C. Sturm, R.H. Austin, Proc. Nat. Acad. Sci. U.S.A. 103, 14779 (2006).

    Google Scholar 

  58. J. Takagi, M. Yamada, M. Yasuda, M. Seki, Lab Chip 5, 778 (2005).

    Google Scholar 

  59. D. Di Carlo, J.F. Edd, D. Irimia, R.G. Tompkins, M. Toner, Anal. Chem. 80, 2204 (2008).

    Google Scholar 

  60. S.S. Shevkoplyas, T. Yoshida, L.L. Munn, M.W. Bitensky, Anal. Chem. 77, 933 (2005).

    Google Scholar 

  61. H. Bow, H.W. Hou, S. Goldfless, P. Abgrall, K.S.W. Tan, J. Niles, C.T. Lim, J. Han, Proceedings of the MicroTAS 2009 conference, Jeju, Korea, pp. 1219 (2009).

    Google Scholar 

  62. S. Suresh, J. Mater. Res. 21 (8), 1871 (2006).

    Google Scholar 

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Authors
  1. Monica Diez-Silva
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  2. Ming Dao
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  3. Jongyoon Han
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  4. Chwee-Teck Lim
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  5. Subra Suresh
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Diez-Silva, M., Dao, M., Han, J. et al. Shape and Biomechanical Characteristics of Human Red Blood Cells in Health and Disease. MRS Bulletin 35, 382–388 (2010). https://doi.org/10.1557/mrs2010.571

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