Abstract
This study investigated the survival and catalytic potential of a single species Pseudomonas taiwanensis VLB120ΔC biofilm for the conversion of styrene to (S)-styrene oxide in a multiphasic capillary microreactor containing the highly toxic substrate styrene as a pure phase. The catalytic biofilm was cultivated under high fluidic stress in a continuous three-phase segmented flow system comprising aqueous medium, air, and styrene. This concept required an adaptation period of 7 days, during which P. taiwanensis VLB120ΔC developed a biofilm exhibiting a remarkable cellular integrity with nearly 70% intact cells. In a three-phase segmented flow biofilm microreactor, an average specific styrene epoxidation rate of 10 g/Ltube/day was achieved continuously for a period of 20 days without any clogging problems. Overall, this note highlights the robustness of biofilms as a promising biocatalyst format for the conversion/synthesis of toxic organic chemicals and the applicability of multiphasic capillary microreactors for biofilm based catalysis.
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Halan, B., Karande, R., Buehler, K. et al. Catalytic Pseudomonas taiwanensis VLB120ΔC Biofilms Thrive in a Continuous Pure Styrene Generated by Multiphasic Segmented Flow in a Capillary Microreactor. J Flow Chem 6, 39–42 (2016). https://doi.org/10.1556/1846.2015.00037
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DOI: https://doi.org/10.1556/1846.2015.00037