Experimental Animals
Online ISSN : 1881-7122
Print ISSN : 1341-1357
ISSN-L : 0007-5124
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Genetic Typing of the Mouse ob Mutation by PCR and Restriction Enzyme Analysis
Tsutomu HIRASAWATadao OHARASusumu MAKINO
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JOURNAL FREE ACCESS

1997 Volume 46 Issue 1 Pages 75-78

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Abstract

A genetic typing method for the mouse obese (ob) mutation by PCR and restriction fragment length polymorphism (RFLP) analysis was developed. Three genotypes (ob/ob, ob/+ and +/+) of the ob mouse are rapidly differentiated with this assay. Since only a small biopsy specimen (tail end) is necessary for the genotyping, the ob mouse at any age is typable and can be used in subsequent breeding or research. Obese homozygotes (ob/ob) were efficiently produced by mating heterozygotes (ob/+) only, which were selected by using the PCR-RFLP assay.

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© 1997 Japanese Association for Laboratory Animal Science
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