Introduction to the Japan Mouse Clinic at the RIKEN BioResource Center

Shigeharu WAKANA; Tomohiro SUZUKI; Tamio FURUSE; Kimio KOBAYASHI; Ikuo MIURA; Hideki KANEDA; Ikuko YAMADA; Hiromi MOTEGI; Hideaki TOKI; Maki INOUE; Osamu MINOWA; Tetsuo NODA; Kazunori WAKI; Nobuhiko TANAKA; Hiroshi MASUYA; Yuichi OBATA

doi:10.1538/expanim.58.443

Review

Introduction to the Japan Mouse Clinic at the RIKEN BioResource Center

Shigeharu WAKANA, Tomohiro SUZUKI, Tamio FURUSE, Kimio KOBAYASHI, Ikuo MIURA, Hideki KANEDA, Ikuko YAMADA, Hiromi MOTEGI, Hideaki TOKI, Maki INOUE, Osamu MINOWA, Tetsuo NODA, Kazunori WAKI, Nobuhiko TANAKA, Hiroshi MASUYA, Yuichi OBATA

Author information

Shigeharu WAKANA
Technology and Development Team for Mouse Phenotype Analysis, RIKEN BioResource Center
Tomohiro SUZUKI
Technology and Development Team for Mouse Phenotype Analysis, RIKEN BioResource Center
Tamio FURUSE
Technology and Development Team for Mouse Phenotype Analysis, RIKEN BioResource Center
Kimio KOBAYASHI
Technology and Development Team for Mouse Phenotype Analysis, RIKEN BioResource Center
Ikuo MIURA
Technology and Development Team for Mouse Phenotype Analysis, RIKEN BioResource Center
Hideki KANEDA
Technology and Development Team for Mouse Phenotype Analysis, RIKEN BioResource Center
Ikuko YAMADA
Technology and Development Team for Mouse Phenotype Analysis, RIKEN BioResource Center
Hiromi MOTEGI
Team for Advanced Development and Evaluation of Human Disease Models, RIKEN BioResource Center
Hideaki TOKI
Team for Advanced Development and Evaluation of Human Disease Models, RIKEN BioResource Center
Maki INOUE
Team for Advanced Development and Evaluation of Human Disease Models, RIKEN BioResource Center
Osamu MINOWA
Team for Advanced Development and Evaluation of Human Disease Models, RIKEN BioResource Center
Tetsuo NODA
Team for Advanced Development and Evaluation of Human Disease Models, RIKEN BioResource Center
Kazunori WAKI
Technology and Development Unit for Knowledge Base of Mouse Phenotype, RIKEN BioResource Center
Nobuhiko TANAKA
Technology and Development Unit for Knowledge Base of Mouse Phenotype, RIKEN BioResource Center
Hiroshi MASUYA
Technology and Development Unit for Knowledge Base of Mouse Phenotype, RIKEN BioResource Center
Yuichi OBATA
RIKEN BioResource Center

Keywords: database, mouse, phenotype, SOPs, standardization

JOURNAL FREE ACCESS

2009 Volume 58 Issue 5 Pages 443-450

DOI https://doi.org/10.1538/expanim.58.443

Details

Abstract

A systematic and comprehensive phenotyping platform has been developed by the RIKEN ENU-mutagenesis project between 1999 and 2007. As a result of phenotype screening on this platform, we have discovered about 400 mutants as animal models for human diseases. All information regarding these mouse mutants is now available to the public through our home page (http://www.brc.riken.jp/lab/gsc/mouse/indexJ.html). In 2008, we reconstructed the existing phenotyping platform and built a new platform. The new system has a hierarchical structure, consisting of a fundamental pipeline that utilizes the existing platform and an additional pipeline, which is optimized for more in-depth phenotyping assays. Using this system, we have started to perform more comprehensive phenotyping of mouse mutants. We have opened this system to Japanese scientists as the Japanese Mouse Clinic. It is anticipated that existing mouse mutants will be reevaluated as disease models by identifying novel phenotypes on the new platform. We will share detailed information about the standard operating procedures (SOPs) of our phenotyping analyses with other related large-scale projects, such as the European Mouse Disease Clinic (EUMODIC) and the German Mouse Clinic (GMC). Moreover, we will contribute to international efforts to standardize mouse phenotype data by sharing annotation of mutant phenotypes, which are made by internationally standardized methods, with other related projects.

Corresponding author

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