1992 Volume 33 Issue 6 Pages 843-850
In order to determine the effect of adenosine triphosphate (ATP) and adenosine in cardioplegic solutions, a comparative study has been undertaken in isolated guinea pig hearts using the Langendorf perfusion technique as a model of cardiopulmonary bypass.
The hearts (n=10 in each group) previously being perfused by Krebs-Henseleit solution, were arrested by one of the following cardioplegic solutions: 1) Potassium 20mM/L (Plegisol), 2) Potassium 20mM/L+ATP 10mM/L, 3) Adenosine 10mM/L, 4) Adenosine 10mM/L+ATP 10mM/L. After 45min of hypothermic ischemia, postischemic recovery of heart rate, ventricular contractility, heart work and postischemic changes in tissue enzymes (LDH, SGOT, SGPT) were com-pared among the 4 different cardioplegic solutions. Arrest time and number of arrest beats were also recorded and compared among the groups.
Although similar beneficial results on postischemic recovery were achieved with adenosine cardioplegia and with ATP supplemented potassium cardioplegia, ATP supplemented adenosine cardioplegia did not show any beneficial effects on postischemic recovery.
