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Induction of erythroid-specific genes by overexpression of GATA-2 in K562 cells

  • Progress in Hematology
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Abstract

GATA transcription factors have been shown to play important roles in hematopoiesis. GATA-2 is expressed in stem and progenitor cells, and has been speculated to control the proliferation and maintain the immaturity of these cells. To examine whether the function of GATA-2 is changeable according to the differentiation stage, we established GATA-2 overexpress-ing subclones of K562, which is a leukemic cell line committed to the erythroid lineage. Via an increase in the GATA-2 expression level, the expression levels of erythroid-specific genes including a-, β-, and γ-globin were increased compared to control cells, while the expression level of GATA-1 was unchanged. Expression of the transferrin receptor was also increased in GATA-2 overexpressing K562 cells when examined by flow cytometry. In addition, the heme content of GATA-2 over-expressing K562 cells was more than 2 times higher than control cells. Chromatin immunoprecipitation analysis showed that GATA-2 protein binding to the GATA element in α-globin LCR was increased in GATA-2 overexpressing K562 cells. These findings suggest that GATA-2 could induce erythroid-specific genes without competition with GATA-1 when expressed in erythroid-committed cells, and thus further suggest that temporal and spatial regulation may be important for displaying specific functions of GATA-2.

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Authors and Affiliations

  1. Department of Rheumatology and Hematology, Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, 980-8574, Aoba-ku, Sendai, Japan

    Hideo Harigae, Yoko Okitsu, Hisayuki Yokoyama, Tohru Fujiwara, Mitsue Inomata & Takeshi Sasaki

  2. Departments of Infection Control and Laboratory Diagnostics, Tohoku University Graduate School of Medicine, Sendai, Japan

    Shinichiro Takahashi & Mitsuo Kaku

  3. Biomedical Engineering Research Organization, Tohoku University Graduate School of Medicine, Sendai, Japan

    Naoko Minegishi

Authors
  1. Hideo Harigae
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  2. Yoko Okitsu
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  3. Hisayuki Yokoyama
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  4. Tohru Fujiwara
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  5. Mitsue Inomata
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  6. Shinichiro Takahashi
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  7. Naoko Minegishi
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  8. Mitsuo Kaku
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  9. Takeshi Sasaki
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Correspondence to Hideo Harigae.

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Harigae, H., Okitsu, Y., Yokoyama, H. et al. Induction of erythroid-specific genes by overexpression of GATA-2 in K562 cells. Int J Hematol 84, 38–42 (2006). https://doi.org/10.1532/IJH97.06020

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  • DOI: https://doi.org/10.1532/IJH97.06020

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