Science for Education Today, 2022, vol. 12, no. 2, pp. 152–171
© Chuenko N. F., Lobkis M. A., Tsybulya N. V., Fershalova T. D., Novikova I. I., 2022
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Evaluating the effectiveness of using phytoncides to reduce microbial contamination of indoor air in order to minimize the risk of illnesses in preschool educational settings

Chuenko N. F. 1 (Novosibirsk, Russian Federation), Lobkis M. A. 1 (Novosibirsk, Russian Federation), Tsybulya N. V. 2 (Novosibirsk, Russian Federation), Fershalova T. D. 2 (Novosibirsk, Russian Federation), Novikova I. I. 1 (Novosibirsk, Russian Federation)
1 Novosibirsk Research Institute of Hygiene of the Federal Service for Supervision of Human Welfare
2 Central Siberian Botanical Garden of the Siberian Branch of the Academy of Sciences
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Abstract: 

Introduction. Due to the high prevalence of respiratory diseases in children attending pre-school educational settings, the problem of their prevention is especially important. Taking into account the fact that children spend between 6 and 8 hours a day in pre-school educational institutions, one of the risk factors of respiratory diseases is the microbial contamination of indoor air. The analysis of Russian and international scholarly literature revealed the positive impact of phytoncide properties of plants on the quality of indoor air and on the psycho-emotional state of a person, however, due to the lack of experimental evidence, the practices of using healing properties of plants in children's organized groups have not been widely adopted. The results of this study confirm that the rational placement of a certain range of plants in preschool classrooms can become a promising and low-cost direction in the structure of a health-saving approach in the modern system of preschool education. The purpose of the study is to evaluate the effectiveness of phytoncides of a particular range of plants and their placement in reducing microbial contamination of the air in pre-school education settings.
Materials and Methods. To study the effect of phytoncide activity of plants the authors monitored the microbial contamination of air in preschool classrooms from two kindergartens in Novosibirsk, with the main focus on the leaf area of the established range of plants and conditions of their placement. For quantitative and qualitative analysis of air microflora composition we used standard differential-diagnostic nutrient media, methods of seeding and calculation of the proportion of total microbial count and facultative microflora. Air sampling points were located in the child's breathing zone (0.5, 1.5 and 3 m away from the plants at 0.8 m height). We monitored the effectiveness of phytoncide properties of
plants on the risks of children's disease during the epidemiological rise based on attendance logs. The following theoretical research methods were used: formalization, generalization, comparison and system analysis.
Results. It was found that phytoncides reduce microbiological insemination of preschool classrooms where a certain range of phytoncide plants were located. It was determined that the intensity of the phytoncide effect depends on the leaf surface area and their rational distribution, taking into account the effective radius of exposure. The study found a decrease in respiratory diseases among children at preschool educational settings where phytoncide plants were placed in the classrooms.
Conclusions. The results of the study can be employed in recommendations for the use of a certain range of plants with pronounced phytoncide activity as one of the components of health-saving conditions in the modern education system.

Keywords: 

Preschool educational settings; Microbial saturation; Total microbial count; Facultative microflora; Phytoncide activity; Indoor plants.

For citation:
Chuenko N. F., Lobkis M. A., Tsybulya N. V., Fershalova T. D., Novikova I. I. Evaluating the effectiveness of using phytoncides to reduce microbial contamination of indoor air in order to minimize the risk of illnesses in preschool educational settings. Science for Education Today, 2022, vol. 12, no. 2, pp. 152–171. DOI: http://dx.doi.org/10.15293/2658-6762.2202.08
References: 
  1. Hwang S. H., Seo S., Yoo Y., Kim K. Y., Choung J. T., Park W. M. Indoor air quality of daycare centers in Seoul, Korea. Building and Environment, 2017, vol. 124, pp. 186–193. DOI: https://doi.org/10.1016/j.buildenv.2017.07.042
  2. Ruggieri S., Longo V., Perrino C., Canepari S., Drago G., L’Abbate L., Balzan M., Cuttitta G., Scaccianoce G., Minardi R., Viegi G. Indoor air quality in schools of a highly polluted south Mediterranean area. Indoor Air, 2019, vol. 29 (2), pp. 276–290. DOI: https://doi.org/10.1111/ina.12529
  3. Carrer P., de Bruin Y. B., Franchi M., Valovirta E. The EFA project: Indoor air quality in European schools. Education, 2002, vol. 2, pp. 794–799. URL: https://www.semanticscholar.org/paper/THE-EFA-PROJECT%3A-INDOOR-AIR-QUALITY-IN-EUROPEAN-Carrer-Bruin/2d51a2f934f3a50984ab513ae0f00078b92fa44c
  4. Kalinina N. V., Gubernsky Yu. D. Risk factors in a residential environment. Hygiene and sanitation, 2002, no. 6, pp. 28-31. URL: https://elibrary.ru/item.asp?id=26676297  
  5. Kalimeri K. K., Saraga D. E., Lazaridis V. D., Legkas N. A., Missia D. A., Tolis E. I., Bartzis J. G. Indoor air quality investigation of the school environment and estimated health risks: two-season measurements in primary schools in Kozani, Greece. Atmospheric Pollution Research, 2016, vol.  7  (6), pp. 1128–1142.  DOI: https://doi.org/10.1016/j.apr.2016.07.002
  6. Brilli F., Fares S., Ghirardo A., de Visser P., Calatayud V., Muñoz A., ... & Menghini, F. Plants for sustainable improvement of indoor air quality. Trends in Plant Science, 2018, vol. 23 (6), pp. 507–512. DOI:  https://doi.org/10.1016/j.tplants.2018.03.004
  7. Kim K. J., Khalekuzzaman M., Suh J. N., Kim H. J., Shagol C., Kim H. H., Kim H. J. Phytoremediation of volatile organic compounds by indoor plants: a review. Horticulture, Environment, and Biotechnology, 2018, vol. 59 (2), pp. 143–157. DOI:  https://doi.org/10.1007/s13580-018-0032-0
  8. Csobod E., Annesi-Maesano I., Carrer P., Kephalopoulos S., Madureira J., Rudnai P. & Viegi G. SINPHONIE Schools Indoor Pollution and Health Observatory Network in Europe Final Report // Co-published by the European Commission's Directorates General for Health and Consumers and Joint Research Centre, Luxembourg. – 2014. DOI: https://doi.org/10.1016/j.scitotenv.2020.139870
  9. Andualem Z., Gizaw Z., Bogale L., Dagne H. Indoor bacterial load and its correlation to physical indoor air quality parameters in public primary schools Multidisciplinary Respiratory Medicine, 2019, vol. 14 (1), pp. 1–7. DOI: https://doi.org/10.1186/s40248-018-0167-y
  10. Lam H. C. Y., Jarvis D., Fuertes E. Interactive effects of allergens and air pollution on respiratory health: a systematic review. Science of the Total Environment, 2021, vol. 757, pp. 143924. DOI: https://doi.org/10.1016/j.scitotenv.2020.143924
  11. Novikova I., Chuenko N., Tsybulya N., Fershalova T., Lobkis M. Quantification of the health-improving action of phyto modules in the rooms of child care preschool facilities. BIO Web of Conferences. – EDP Sciences, 2021, vol. 38. DOI:  https://doi.org/10.1051/bioconf/20213800091
  12. Kim C., Choi D., Lee Y. G., Kim K. Diagnosis of indoor air contaminants in a daycare center using a long-term monitoring. Building and Environment, 2021, vol. 204, pp. 108124. DOI: https://doi.org/10.1016/j.buildenv.2021.108124
  13. Timofeeva S. С. Modern phytotechnologies of air purification. Part 1. Technologies of air purification in closed rooms: medical and ecological phytodesign. The XXI Century. Technosphere Safety, 2017, vol. 2 (1), pp. 55–69. (In Russian) URL: https://www.elibrary.ru/item.asp?id=28844588 
  14. Dukhanov S. С. Standardized design in Western Siberia late in the 1950-60s. Bulletin of the Tomsk State Architectural and Construction University, 2021, vol. 23 (1), pp. 19–33. (In Russian)  DOI: https://doi.org/10.31675/1607-1859-2021-23-1-19-33 URL: https://www.elibrary.ru/item.asp?id=44754318
  15. Turchakova A. S., Tkachenko N. V. Problems of providing microclimate systems in medical and preventive institutions.  New ideas of the new century: Proceedings of the International Scientific Conference of FAD TOGU, 2021, vol. 3, pp. 412–416. (In Russian) URL: https://www.elibrary.ru/item.asp?id=46181501
  16. Dolbilova M. A., Popova N. M. Features of the organization of natural ventilation in educational institutions. Gradostroitelstvo. Infrastructure. Communications, 2021, no. 1, pp. 39–43. (In Russian) URL: https://www.elibrary.ru/item.asp?id=44903775 
  17. Branco P. T., Alvim-Ferraz M. C., Martins F. G., Ferraz C., Vaz L. G., Sousa S. I. Impact of indoor air pollution in nursery and primary schools on childhood asthma. Science of the Total Environment, 2020, vol. 745, pp. 140982. DOI: https://doi.org/10.1016/j.scitotenv.2020.140982
  18. Basińska M., Michałkiewicz M., Ratajczak K. Impact of physical and microbiological parameters on proper indoor air quality in nursery. Environment International, 2019, vol. 132, pp. 105098. DOI:  https://doi.org/10.1016/j.envint.2019.105098
  19. Anoshkina E. V., Gammel I. V., Kononova S. V. Respiratory disease incidence dynamics in children of our country. Medical Almanac, 2018, no. 3, pp. 120–123. (In Russian) URL: https://www.elibrary.ru/item.asp?id=34923693
  20. Chegini F. M., Baghani A. N., Hassanvand M. S., Sorooshian A., Golbaz S., Bakhtiari R., Ashouri A., Joubani M. N., Alimohammadi M. Indoor and outdoor airborne bacterial and fungal air quality in kindergartens: Seasonal distribution, genera, levels, and factors influencing their concentration. Building and Environment, 2020, vol. 175, pp. 106690. DOI: https://doi.org/10.1016/j.buildenv.2020.106690
  21. Prasher P., Sharma M., Mehta M., Paudel K. R., Satija S., Chellappan D. K., ... Dua K. Plants derived therapeutic strategies targeting chronic respiratory diseases: Chemical and immunological perspective. Chemico-Biological Interaction, 2020, vol. 325, pp. 109125. DOI: https://doi.org/10.1016/j.cbi.2020.109125
  22. Badyda A. J., Dqbrowiecki P., Czechowski P. O., Majewski G. Risk of bronchi obstruction among non-smokers – Review of environmental factors affecting bronchoconstriction. Respiratory Physiology & Neurobiology, 2015, vol. 209, pp. 39–46. DOI: https://doi.org/10.1016/j.resp.2014.10.016
  23. Zhai L., Zhao J., Xu B., Deng Y., Xu Z. Influence of indoor formaldehyde pollution on respiratory system health in the urban area of Shenyang, China. African Health Sciences, 2013, vol. 13 (1), pp.  137–143. DOI:  https://doi.org/10.4314/ahs.v13i1.19
  24. Goldizen F. C., Sly P. D., Knibbs L. D. Respiratory effects of air pollution on children. Pediatric Pulmonology, 2016, vol. 51 (1), pp. 94–108. DOI:  https://doi.org/10.1002/ppul.23262
  25. Agarkov N. M., Poshibailova A. V., Ivanov V. A. Atmospheric pollutants and prevalence of asthma among children: A review. Human Ecology, 2020, no. 5, pp. 45–49. (In Russian) DOI: https://doi.org/10.33396/1728-0869-2020-5-45-49 URL: https://www.elibrary.ru/item.asp?id=42820310
  26. Valina S. L., Zaitseva N. V., Shtina I. E., Ustinova O. Yu., Eisfeld D. A. Hygienic assessment of impacts exerted by factors related to educational process and lifestyle on health of schoolchildren attending secondary schools in industrial megacity. Hygiene and Sanitation, 2020, vol. 99 (8), pp. 822–828. (In Russian) DOI: https://doi.org/10.47470/0016-9900-2020-99-8-822-828 URL: https://www.elibrary.ru/item.asp?id=43918279
  27. Yakimova Yu. L., Rychkova N. A., Tsybulya N. V. Ecological and medical phytodesign as a method of collective health improvement in children's institutions. Siberian Ecological Journal, 2002, Vol. 9 (2), pp. 249-253. URL: https://www.elibrary.ru/item.asp?id=22654516
  28. Wolverton B. C., McDonald R. C., Mesick H. H. Foliage plants for indoor removal of the primary combustion gases carbon monoxide and nitrogen dioxide. Environmental Science, 1985. URL: https://www.semanticscholar.org/paper/Foliage-plants-for-indoor-removal-of-the-primary-Wolverton-Mcdonald/818e0d809148d0141d6fc5fb747210d28e8b5e75
  29. Krestinina N. V., Nekrasova М. А. Improving aspects of the indoor space gardening of classroom. Bulletin of the Peoples' Friendship University of Russia. Series: Ecology and Life Safety, 2007, no.  4, pp. 13-15. URL: https://www.elibrary.ru/item.asp?id=12796086
  30. Chubatova S. A. Phytoncides: History and application prospects. Bacteriology, 2020, vol. 5 (3), pp. 60–67. (In Russian) DOI: https://doi.org/10.20953/2500-1027-2020-3-60-67 URL: https://www.elibrary.ru/item.asp?id=45705372
  31. Deng L., Deng Q. The basic roles of indoor plants in human health and comfort. Environmental Science and Pollution Research, 2018, vol. 25 (36), pp. 36087–36101. DOI: https://doi.org/10.1007/s11356-018-3554-1
  32. Jung C., Awad J. Improving the IAQ for learning efficiency with indoor plants in university classrooms in Ajman, United Arab Emirates. Buildings, 2021, vol. 11 (7), pp. 289. DOI: https://doi.org/10.3390/buildings11070289
  33. Tsybulia N. V., Fershalova T. D. Seasonal antimicrobial activity of volatile substances emitted by the representatives of begonia l. Genus (begoniaceae). Samara Scientific Bulletin, 2021, vol. 10 (1), pp. 167–172. (In Russian) DOI: https://doi.org/10.17816/snv2021101126 URL: https://www.elibrary.ru/item.asp?id=45765135
  34. Peng Z., Deng W., Hong Y., Chen Y. An experimental work to investigate the capabilities of plants to remove particulate matters in an enclosed greenhouse. Air Quality, Atmosphere & Health, 2020, vol. 13 (4), pp. 477–488. DOI: https://doi.org/10.1007/s11869-020-00806-w
  35. Tsybulia N. V., Fershalova T. D., Yakimova Y. L. Role of medical and ecological phytodesign in the restoration of indoor air guality in children''s institutions. Disinfection Business, 2018, no. 1, pp. 31–36. (In Russian) URL: https://www.elibrary.ru/item.asp?id=32615644 
  36. Belyaev A. L., Feodoritova E. L. Problems of epidemiology and prevention of influenza and acute respiratory infections. Quality Management in Healthcare, 2017, no. 3, pp. 4–10. (In Russian) URL: https://www.elibrary.ru/item.asp?id=29947752 
  37. Saulova T. A., Bas V. I. Fitoionization in the systems of ecodesign. Reshetnev Readings, 2017, vol.  2, pp. 108–109. (In Russian) URL: https://www.elibrary.ru/item.asp?id=32291008
  38. Bagaeva O. I., Rogov V. A. Analysis of methods of improvement of microclimate in premises. Actual Problems of Aviation and Cosmonautics, 2018, vol. 2 (4), pp. 501–503. (In Russian) URL: https://www.elibrary.ru/item.asp?id=36804865 
  39. Feklisova L. V., Elezova L. I. Reduction of incidence of acute respiratory infections among children in sanatorium facilities: Reconceptualization. Treatment and Prevention, 2017, no. 2, pp. 93–100. (In Russian) URL: https://www.elibrary.ru/item.asp?id=29932911 
  40. Timofeeva S. С. Phytomining: Current state and prospects. XXI century. Technosphere Safety, 2018, vol. 3 (3), pp. 112–128. (In Russian) DOI: https://doi.org/10.21285/1814-3520-2018-3-112-128 URL: https://www.elibrary.ru/item.asp?id=35639331
  41. Znamenskaya T. K., Vorobyova O. V. Modern aspects of prevention and treatment of influenza and ARVI in children. Sovremennaya Pediatriya, 2017, no 6, pp. 98–104. (In Russian) DOI: https://doi.org/10.15574/SP.2017.86.98 URL: https://www.elibrary.ru/item.asp?id=32388823
  42. Sergeeva I. V., Yamshchikov A. S., Debelova T. A. Aeration of premises by means of protection against respiratory infections on the basis of natural fitoncydes in the complex of prevention of influenza and sars in the conditions of the collectives of preschool and school heats concerns. Medical Board, 2019, no. 11, pp. 67–73. (In Russian) DOI: https://doi.org/10.21518/2079-701X-2019-11-67-73 URL: https://www.elibrary.ru/item.asp?id=38570680
  43. Tsybulia N. V., Fershalova T. D., Davidovich L. A. Use the tropical plants for air sanitation in room ecologically adverse conditions. Izvestia of Samara Scientific Center of the Russian Academy of Sciences, 2017, vol. 19 (2–2), pp. 360–364. (In Russian) URL: https://www.elibrary.ru/item.asp?id=29945996
  44. Zhang S., Mumovic D., Stamp S., Curran K., Cooper E. What do we know about indoor air quality of nurseries? A review of the literature. Building Services Engineering Research and Technology, 2021, vol. 42 (5), pp. 603–632. DOI: https://doi.org/10.1177/01436244211009829   
Date of the publication 30.04.2022