The mating pheromone of baker’s yeast, the a-factor, is a dodeca-/tridecapeptide, which is not antigenic by itself. It was coupled to succinylated thyroglobulin by the carbodiimide procedure to facilitate selective coupling of the a-factor mainly by its N-terminal region. Antibodies against this conjugate were raised in rabbits. After selective precipitation of the rabbit antiserum with succinylated carrier prior to the radial double diffusion test (Ouchterlony) specific antibodies against the coupled a-factor could be detected
Received: 1983-9-5
Revised: 1983-4-21
Published Online: 2014-6-2
Published in Print: 1983-12-1
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