Abstract
Rhodopseudomonas capsulata E1F1 bacteria photo-assimilated nitrate under anaerobic conditions, but were incapable of reducing it in the dark. The bacteria utilized dissolved N2 as nitrogen source, but the growth in the presence of nitrate was 5-fold higher. Nitrite was excreted to the medium, but not stoichiometrically with respect to nitrate consumption. Nitrate reductase is particulate and used reduced viologens or flavins as electron donors for nitrate reduction. The enzyme was inhibited in vitro by KCN, NaN3, dithioerythritol and mercurials. Ammonia and other sources of reduced nitrogen repressed nitrate reductase synthesis.
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