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BY-NC-ND 3.0 license Open Access Published by De Gruyter June 2, 2014

Die quantitative Bestimmung von Chloroplastenpigmenten im picomol-Bereich mit Hilfe einer isochratischen HPLC-Methode/ A Method for the Quantitative Estimation of Chloroplast Pigments in the Picomole-Range by Use of a Isocratic HPLC-System

  • Harald Stransky
From the journal Zeitschrift für Naturforschung C
https://doi.org/10.1515/znc-1978-11-1206
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Abstract

A method is described for the estimation of the pigment content in chloroplasts by means of HPLC. The method is highly sensitive. Less than one picomole of individual pigments - equivalent to 1 milligramme fresh weight of spinach leaves - is needed for quantitative analysis. Analysis time is only 15 min, whereas in routine determination by thin layer chromatography a single sample needs 4 hours to be completed.

Moreover the pigments are protected against desintegration by light and air. Using a isocratic HPLC -method no equilibration of coloumns is necessary, thus permitting four runs per hour.

Keywords: Carotenoids; Chloroplast Pigments; High Performance Liquid Chromatography
Received: 1978-10-16
Published Online: 2014-6-2
Published in Print: 1978-12-1

© 1946 – 2014: Verlag der Zeitschrift für Naturforschung

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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From the journal
Zeitschrift für Naturforschung C
Zeitschrift für Naturforschung C
Volume 33 Issue 11-12
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Subject Index: Original Communications and Notes
Authors Index
Contents
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