Abstract
Objectives
Arginine-stimulated serum copeptin has been proposed as a new method to diagnose arginine vasopressin (AVP) deficiency in children and adolescents. Herein we investigated the secretagogic potential of clonidine or L-Dopa on the copeptin serum levels in children.
Methods
Eight stimulation tests (4 with clonidine and 4 with L-Dopa) were performed in eight children (5 boys and 3 girls) with a median age of 6.5 years-old, evaluated for short stature due to possible growth hormone deficiency. Serum copeptin levels were measured at 30, 60, 90, and 120 min after administration of clonidine or L-Dopa.
Results
Copeptin levels in serum did not show any significant change in either test (clonidine or L-Dopa). The values of copeptin levels compared to the baseline value did not deviate more than 5 % in the clonidine arm (p=0.60) or 8 % in the L-Dopa arm (p=0.75) respectively.
Conclusions
Data do not support the use of L-Dopa or clonidine as stimulants for evaluating AVP relating disorders in clinical pediatric practice.
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Research ethics: The study was carried out in accordance with the Declaration of Helsinki and approved by the local Ethics Committee, University Hospital of Patras.
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Informed consent: Informed consent was obtained from all individuals included in this study, or their legal guardians or wards.
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Author contributions: AG wrote the manuscript, DK processed the samples, DC conceived and critically received the manuscript. The authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: The authors state no conflict of interest.
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Research funding: None declared.
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Data availability: The raw data can be obtained on request from the corresponding author.
References
1. Refardt, J, Winzeler, B, Christ-Crain, M. Diabetes insipidus an update. Metab Clin 2020;49:517–31.10.1016/j.ecl.2020.05.012Search in Google Scholar PubMed
2. Dabrowski, E, Kadakia, R, Zimmerman, D. Diabetes insipidus in infants and children. Best practice & research. Clin Endocrinol Metab 2016;30:317–28. https://doi.org/10.1016/j.beem.2016.02.006.Search in Google Scholar PubMed
3. Cadnapaphornchai, MA, Summer, SN, Falk, S, Thurman, JM, Knepper, MA, Schrier, RW. Effect of primary polydipsia on aquaporin and sodium transporter abundance. Am J Physiol 2003;285:F965–71.10.1152/ajprenal.00085.2003Search in Google Scholar PubMed
4. Kluge, M, Riedl, S, Erhart-Hofmann, B, Hartmann, J, Waldhauser, F. Improved extraction procedure and RIA for determination of arginine8-vasopressin in plasma: role of premeasurement sample treatment and reference values in children. Clin Chem 1999;45:98–103.10.1093/clinchem/45.1.98Search in Google Scholar
5. Fenske, W, Refardt, J, Christ-Crain, M. Copeptin in the diagnosis of diabetes insipidus. N Engl J Med 2018;379:1784–6.10.1056/NEJMc1811694Search in Google Scholar PubMed
6. Balanescu, S, Kopp, P, Gaskill, MB, Morgenthaler, NG, Schindler, C, Rutishauser, J. Correlation of plasma copeptin and vasopressin concentrations in hypo-, iso-, and hyperosmolar states. J Clin Endocrinol Metab 2011;96:1046–52.10.1210/jc.2010-2499Search in Google Scholar PubMed
7. Fenske, WK, Schnyder, I, Koch, G, Walti, C, Pfister, M, Kopp, P, et al.. Release and decay kinetics of copeptin vs AVP in response to osmotic alterations in healthy volunteers. J Clin Endocrinol Metab 2017;103:505–13.10.1210/jc.2017-01891Search in Google Scholar PubMed
8. Winzeler, B, Cesana-Nigro, N, Refardt, J, Vogt, DR, Imber, C, Morin, B, et al.. Arginine-stimulated copeptin measurements in the differential diagnosis of diabetes insipidus: a prospective diagnostic study. Lancet 2019;394:587–95. https://doi.org/10.1016/s0140-6736(19)31255-3.Search in Google Scholar PubMed
9. Bonnet, L, Marquant, E, Fromonot, J, Hamouda, I, Berbis, J, Godefroy, A, et al.. Copeptin assays in children for the differential diagnosis of polyuria-polydipsia syndrome and reference levels in hospitalized children. Clin Endocrinol 2022;96:47–53. https://doi.org/10.1111/cen.14620.Search in Google Scholar PubMed
10. Refardt, J, Atila, C, Chifu, I, Ferrante, E, Erlic, Z, Drummond, JB, et al.. Arginine or hypertonic saline-stimulated copeptin to diagnose AVP deficiency. N Engl J Med 2023;389:1877–87. https://doi.org/10.1056/nejmoa2306263.Search in Google Scholar PubMed
11. Iványi, T, Laczi, F, Kovács, GL, Szabó, G, Janáky, T, Telegdy, G, et al.. Stimulatory effect of intracerebroventricularly administered dopamine on vasopressin release in rats. Exp Clin Endocrinol 1986;88:303–8.10.1055/s-0029-1210610Search in Google Scholar PubMed
12. Nagyéri, G, Gálfi, M, Radács, M, Molnár, AH, László, F, Varga, C, et al.. Effects of galanin–monoaminergic interactions on vasopressin secretion in rat neurohypophyseal cell cultures. Regul Pept 2009;155:76–80. https://doi.org/10.1016/j.regpep.2009.03.004.Search in Google Scholar PubMed
13. Knigge, U, Willems, E, Kjær, A, Jørgensen, H, Warberg, J. Histaminergic and catecholaminergic interactions in the central regulation of vasopressin and oxytocin secretion. Endocrinology 1999;140:3713–9.10.1210/endo.140.8.6891Search in Google Scholar PubMed
14. Zhaoge, B, Chapman, C, Brown, D, Bicknell, J. Opioid-noradrenergic interactions in the neurohypophysis. Neuroendocrinology 1988;48:25–31. https://doi.org/10.1159/000124985.Search in Google Scholar PubMed
15. Wales, JK. Evaluation of growth disorders. In: Brook CG, Clayton PE, Brown RS, editors. Brook’s clinical pediatric endocrinology. West Sussex: John Wiley and Sons Ltd; 2009:124–54 pp.Search in Google Scholar
16. Pelletier, G. Identification of ending containing dopamine and vasopressin in the rat posterior pituitary by a combination of radioautography and immunocytochemistry at the ultrastructural level. J Histochem Cytochem 1983;31:562–4. https://doi.org/10.1177/31.4.6827086.Search in Google Scholar PubMed
17. Scantamburlo, G, Hansenne, M, Fuchs, S, Pitchot, W, Pinto, E, Reggers, J, et al.. AVP- and OT-response to apomorphine and clonidine in major depression. Psychoneuroendocrinology 2005;30:839–45.10.1016/j.psyneuen.2005.04.015Search in Google Scholar PubMed
18. Livingston, A, Morris, B. Localisation of [3H] clonidine binding in rat neurohypophysis by means of electron-microscopic autoradiography. Tissue Res 1986;244:467–9.10.1007/BF00219223Search in Google Scholar PubMed
19. Armstrong, WE, Sladek, CD, Sladek, JR. Characterization of noradrenergic control of vasopressin release by the organ-cultured rat hypothalamo-neurohypophyseal system. Endocrinology 1982;111:273–9.10.1210/endo-111-1-273Search in Google Scholar PubMed
20. Sailer, CO, Refardt, J, Blum, CA, Schnyder, I, Molina-Tijeras, JA, Fenske, W, et al.. Validity of different copeptin assays in the differential diagnosis of the polyuria-polydipsia syndrome. Sci Rep 2021;11:10104. https://doi.org/10.1038/s41598-021-89505-9.Search in Google Scholar PubMed PubMed Central
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