Abstract
Background
Myrtle (Myrtus communis L) may constitute an interesting dietary source of health protective compounds. Microwave-assisted extraction (MAE) of total phenolic compounds (TPC) from myrtle leaf, stems, pericarp, and seeds was studied and the results were compared with those of the conventional method extraction (CME) in terms of extraction time.
Methods
Extraction yield/efficiency and antioxidant activity were measured using radical scavenging assay (DPPH•) and reducing power.
Results
The results show that the MAE was higher in terms of saving energy, extraction time (62 s) and extraction efficiency of bioactive compound compared to CME (2 h). Leaf presented the optimum content of total phenols (250 mg GAE.g−1 DW) and flavonoids (13.65 mg GAE.g−1 DW). However, the anthocyanin content was most important in pericarp extract (176.50±2.17 mg Cyd-3-glu g−1 DW). The antioxidant activity was important in all parts, mainly in leaves. The results indicated that appropriate microwave treatment could be an efficient process to phenolic compounds recovery and thus, better the antioxidant activity of myrtle extract.
Conclusions
Principal component analysis (PCA) applied to the experimental data shows that the distribution of the myrtle phenolic compounds depended on their plant part localization as well as the extraction method.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
References
1. Aydın C, Özcan MM. Determination of nutritional and physical properties of myrtle (Myrtus communis L.) fruits growing wild in Turkey. J Food Eng. 2007;79:453–458.10.1016/j.jfoodeng.2006.02.008Search in Google Scholar
2. Chalchat J-C, Garry R-P, Michet A. Essential oils of myrtle (Myrtus communis L.) of the Mediterranean littoral. J Essent Oil Res. 1998;10:613–617.10.1080/10412905.1998.9700988Search in Google Scholar
3. Marchini G, Maccioni S. Liguria in parole povere. La bassa Val di Magra. Genova: Sagep, 1998.Search in Google Scholar
4. Messaoud C, Zaouali Y, Salah AB, Khoudja ML, Boussaid M. Myrtus communis in Tunisia: Variability of the essential oil composition in natural populations. Flavour Frag J. 2005;20:577–582.10.1002/ffj.1490Search in Google Scholar
5. Aidi Wannes W, Marzouk B. Differences between myrtle fruit parts (Myrtus communis var. italica) in phenolics and antioxidant contents. J Food Biochem. 2013;37:585–594.10.1111/jfbc.12010Search in Google Scholar
6. Messaoud C, Boussaid M. Myrtus communis Berry Color morphs: a comparative analysis of essential oils, fatty acids, phenolic compounds, and antioxidant activities. Chem Biodivers. 2011;8:300–310.10.1002/cbdv.201000088Search in Google Scholar PubMed
7. Aspé E, Fernández K. The effect of different extraction techniques on extraction yield, total phenolic, and anti-radical capacity of extracts from Pinus radiata Bark. Ind Crops Prod. 2011;34:838–844.10.1016/j.indcrop.2011.02.002Search in Google Scholar
8. Dahmoune F, Boulekbache L, Moussi K, Aoun O, Spigno G, Madani K. Valorization of Citrus limon residues for the recovery of antioxidants: evaluation and optimization of microwave and ultrasound application to solvent extraction. Ind Crops Prod. 2013;50:77–87.10.1016/j.indcrop.2013.07.013Search in Google Scholar
9. Jun X, Deji S, Ye L, Rui Z. Comparison of in vitro antioxidant activities and bioactive components of green tea extracts by different extraction methods. Int J Pharm. 2011;408:97–101.10.1016/j.ijpharm.2011.02.002Search in Google Scholar PubMed
10. Luque De Castro MD, Garcı́a-Ayuso LE. Soxhlet extraction of solid materials: An outdated technique with a promising innovative future. Anal Chim Acta. 1998;369:1–10.10.1016/S0003-2670(98)00233-5Search in Google Scholar
11. Yang Z, Zhai W. Optimization of microwave-assisted extraction of anthocyanins from purple corn (Zea mays L.) cob and identification with HPLC–MS. Innovative Food Sci Emerg Technol. 2010;11:470–476.10.1016/j.ifset.2010.03.003Search in Google Scholar
12. Amensour M, Sendra E, Abrini J, Pérez-Alvarez JA, Fernández-López J. Antioxidant activity and total phenolic compounds of myrtle extracts actividad antioxidante y contenido de compuestos fenólicos totales en extractos de myrtus. Cyta – J Food. 2010;8:95–101.10.1080/19476330903161335Search in Google Scholar
13. Dahmoune F, Nayak B, Moussi K, Remini H, Madani K. Optimization of microwave-assisted extraction of polyphenols from Myrtus communis L. leaves. Food Chem. 2015;166:585–595.10.1016/j.foodchem.2014.06.066Search in Google Scholar PubMed
14. George S, Brat P, Alter P, Amiot MJ. Rapid determination of polyphenols and vitamin c in plant-derived products. J Agric Food Chem. 2005;53:1370–1373.10.1021/jf048396bSearch in Google Scholar PubMed
15. Quettier-Deleu C, Gressier B, Vasseur J, Dine T, Brunet C, Luyckx M, et al. Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. J Ethnopharmacol. 2000;72:35–42.10.1016/S0378-8741(00)00196-3Search in Google Scholar PubMed
16. Lee J, Durst RW, Wrolstad RE. Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: Collaborative study. J AOAC Int. 2005;88:1269–1278.10.1093/jaoac/88.5.1269Search in Google Scholar
17. Aidi Wannes W, Mhamdi B, Sriti J, Ben Jemia M, Ouchikh O, Hamdaoui G, et al. Antioxidant activities of the essential oils and methanol extracts from myrtle (Myrtus communis var. italica L.) leaf, stem and flower. Food Chem Toxicol. 2010;48:1362–1370.10.1016/j.fct.2010.03.002Search in Google Scholar PubMed
18. Gülçin İ, Mshvildadze V, Gepdiremen A, Elias R. Screening of antiradical and antioxidant activity of monodesmosides and crude extract from Leontice smirnowii tuber. Phytomedicine. 2006;13:343–351.10.1016/j.phymed.2005.03.009Search in Google Scholar PubMed
19. Choi CW, Kim SC, Hwang SS, Choi BK, Ahn HJ, Lee MY, et al. Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay-guided comparison. Plant Sci. 2002;163:1161–1168.10.1016/S0168-9452(02)00332-1Search in Google Scholar
20. Dudonné S, Vitrac X, Coutière P, Woillez M, Mérillon J-M. Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays. J Agric Food Chem. 2009;57:1768–1774.10.1021/jf803011rSearch in Google Scholar PubMed
21. Zou Y, Lu Y, Wei D. Antioxidant activity of a flavonoid-rich extract of hypericum perforatum L. in Vitro. J Agric Food Chem. 2004;52:5032–5039.10.1021/jf049571rSearch in Google Scholar PubMed
22. Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, Vidal N. Antioxidant activity of some algerian medicinal plants extracts containing phenolic compounds. Food Chem. 2006;97:654–660.10.1016/j.foodchem.2005.04.028Search in Google Scholar
23. Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem. 2001;49:5165–5170.10.1021/jf010697nSearch in Google Scholar PubMed
24. Gardeli C, Vassiliki P, Athanasios M, Kibouris T, Komaitis M. Essential oil composition of Pistacia lentiscus L. and Myrtus communis L.: evaluation of antioxidant capacity of methanolic extracts. Food Chem. 2008;107:1120–1130.10.1016/j.foodchem.2007.09.036Search in Google Scholar
25. Piras FM, Dettori MF, A. M. ToF-SIMS PCA analysis of Myrtus communis L. Appl Surf Sci. 2009;255:7805–7811.10.1016/j.apsusc.2009.04.183Search in Google Scholar
26. Prior RL, Cao G. In vivo total antioxidant capacity: Comparison of different analytical methods1. Free Radical Biol Med. 1999;27:1173–1181.10.1016/S0891-5849(99)00203-8Search in Google Scholar
27. Cheung LM, Cheung PC, Ooi VE. Antioxidant activity and total phenolics of edible mushroom extracts. Food Chem. 2003;81:249–255.10.1016/S0308-8146(02)00419-3Search in Google Scholar
28. Mandal V, Mohan Y, Hemalatha S. Microwave assisted extraction-an innovative and promising extraction tool for medicinal plant research. Pharmacogn Rev. 2007;1:7.Search in Google Scholar
29. S-F JIA, H-X DONG, S-T DONG. Optimization of ultrasound-assisted extraction of anthocyan from purple maize. Food Nutr China. 2011;2:015.Search in Google Scholar
30. Montoro P, Tuberoso CI, Piacente S, Perrone A, De Feo V, Cabras P, et al. Stability and antioxidant activity of polyphenols in extracts of Myrtus communis L. berries used for the preparation of myrtle liqueur. J Pharm Biomed Anal. 2006;41:1614–1619.10.1016/j.jpba.2006.02.018Search in Google Scholar PubMed
31. Canhoto J, Lopes M, Cruz G.. In vitro propagation of Myrtus communis through somatic embryogenesis and axillary shoot proliferation. Abstract Book of 1st International Meeting of Aromatic and Medicinal Mediterranean Plants, 1998.Search in Google Scholar
32. Pérez-Serradilla JA, Luque De Castro MD. Microwave-assisted extraction of phenolic compounds from wine lees and spray-drying of the extract. Food Chem. 2011;124:1652–1659.10.1016/j.foodchem.2010.07.046Search in Google Scholar
33. Proestos C, Komaitis M. Application of microwave-assisted extraction to the fast extraction of plant phenolic compounds. LWT – Food Sci Technol. 2008;41:652–659.10.1016/j.lwt.2007.04.013Search in Google Scholar
34. Chen Y, Xie M-Y, Nie S-P, Li C, Wang Y-X. Purification, composition analysis and antioxidant activity of a polysaccharide from the fruiting bodies of Ganoderma atrum. Food Chem. 2008;107:231–241.10.1016/j.foodchem.2007.08.021Search in Google Scholar
35. Zhou H-Y, Liu C-Z. Microwave-assisted extraction of solanesol from tobacco leaves. J Chromatogr A. 2006;1129:135–139.10.1016/j.chroma.2006.07.083Search in Google Scholar PubMed
36. Ferchichi L, Le Ray A, Guilet D, Litaudon M, Awangt K, Hadi A, et al. Bio-active secondary metabolites from two Malaysian clusaceae: calophyllum flavo-ramulum and C. wallichianum. Planta Med. 2009;75:32.10.1055/s-0029-1234357Search in Google Scholar
37. Yoshimura M, Amakura Y, Tokuhara M, Yoshida T. Polyphenolic compounds isolated from the leaves of Myrtus communis. J Nat Med. 2008;62:366–368.10.1007/s11418-008-0251-2Search in Google Scholar PubMed
38. Chou S-T, Chiang B-H, Chung Y-C, Chen P-C, Hsu C-K. Effects of storage temperatures on the antioxidative activity and composition of yam. Food Chem. 2006;98:618–623.10.1016/j.foodchem.2005.06.039Search in Google Scholar
39. Pan Y, He C, Wang H, Ji X, Wang K, Liu P. Antioxidant activity of microwave-assisted extract of Buddleia officinalis and its major active component. Food Chem. 2010;121:497–502.10.1016/j.foodchem.2009.12.072Search in Google Scholar
40. Simopoulos AP. Omega-3 fatty acids and antioxidants in edible wild plants. Biol Res. 2004;37:263–277.10.4067/S0716-97602004000200013Search in Google Scholar PubMed
41. Aa O, Gomez JD, Cudmani NM, Vattuone MA, Isla MI. Antimicrobial activity of nine extracts of Sechium edule (Jacq.) Swartz. Microb Ecol Health Dis. 2003;15:33–39.10.1080/0891060010015583Search in Google Scholar
42. Sengul M, Yildiz H, Gungor N, Cetin B, Eser Z, Ercisli S. Total phenolic content, antioxidant and antimicrobial activities of some medicinal plants. Pak J Pharm Sci. 2009;22:102–106.Search in Google Scholar PubMed
43. Chiang C-J, Kadouh H, Zhou K. Phenolic compounds and antioxidant properties of gooseberry as affected by in vitro digestion. LWT – Food Sci Technol. 2013;51:417–422.10.1016/j.lwt.2012.11.014Search in Google Scholar
44. Zhang B, Yang R, Liu C-Z. Microwave-assisted extraction of chlorogenic acid from flower buds of Lonicera japonica thunb. Sep Purif Technol. 2008;62:480–483.10.1016/j.seppur.2008.02.013Search in Google Scholar
45. Hayat K, Zhang X, Chen H, Xia S, Jia C, Zhong F. Liberation and separation of phenolic compounds from citrus mandarin peels by microwave heating and its effect on antioxidant activity. Sep Purif Technol. 2010;73:371–376.10.1016/j.seppur.2010.04.026Search in Google Scholar
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