Abstract
Objectives
Cistus salviifoluis L. is a shrub from Cistaceae family used in many traditional medicines for the treatment of various diseases including diabetes mellitus. The aim of this study was to evaluate the in vivo antidiabetic potential of the aerial parts aqueous extract of Cistus salviifolius L. (CSA).
Methods
Experimental diabetes was induced in adult male mice by intra-peritoneal injection of Streptozotocin-nicotinamide (STZ-NC). CSA at a dose of 500 mg/kg was administered daily to the diabetic mice for four weeks. The effect of the extract on hyperglycemia, body weight, serum total cholesterol, triglycerides, hepatic and renal functional markers were determined. Histopathological examination of the mice pancreas was also performed. The diabetic animals treated with CSA were compared with animals treated by the standard drug metformin.
Results
Treatment with CSA showed a significant reduction in blood glucose, total triglycerides and creatinine levels and prevented the reduction of body weight caused by diabetes. Furthermore, histopathological analysis of the mice pancreas showed that the group treated with CSA reduced damage induced by STZ-NC on islets of Langerhans cells when compared to the diabetic control.
Conclusions
The results suggest that the aqueous extract of Moroccan C. salviifolius L. possesses beneficial effect on treatment of diabetes.
Acknowledgments
The authors are thankful to Pr. Soufiane Derraji (Faculty of Medicine and Pharmacy of Rabat, Morocco) and Dr. Zohra Ouzzif (Biochemistry Department, Military Hospital Mohammed V, Rabat, Morocco) for their support in biochemical analysis, and to Dr. Ilias Marmouzi (Faculty of Medicine and Pharmacy of Rabat, Morocco) for his assistance in the laboratory. We are also thankful to Pr. Halim Khamar (Botanical Department of the Scientific Institute of Rabat, Morocco) for plant authentication.
Research funding: None declared.
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no conflict of interest.
Informed consent: Informed consent was obtained from all individuals included in this study.
Ethical approval: The recommendations described in the Guide for the Care and Use of Laboratory Animals, prepared by the National Academy of Sciences and published by the National Institutes of Health were followed in the experimental procedure. Ethics approval was obtained from the University Mohammed V in Rabat, Morocco.
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