Change language
English Deutsch
Change currency
€ EUR £ GBP $ USD
Licensed Unlicensed Requires Authentication Published by De Gruyter September 11, 2008

Immunohistochemical evidence of ubiquitous distribution of the metalloendoprotease insulin-degrading enzyme (IDE; insulysin) in human non-malignant tissues and tumor cell lines

  • Gregor Weirich , Karin Mengele , Christina Yfanti , Apostolos Gkazepis , Daniela Hellmann , Anita Welk , Cecylia Giersig , Wen-Liang Kuo , Marsha Rich Rosner , Wei-Jen Tang and Manfred Schmitt
From the journal Biological Chemistry
https://doi.org/10.1515/BC.2008.157

Abstract

Immunohistochemical evidence of ubiquitous distribution of the metalloprotease insulin-degrading enzyme (IDE; insulysin) in human non-malignant tissues and tumor cells is presented. Immunohistochemical staining was performed on a multi-organ tissue microarray (pancreas, lung, kidney, central/peripheral nervous system, liver, breast, placenta, myocardium, striated muscle, bone marrow, thymus, and spleen) and on a cell microarray of 31 tumor cell lines of different origin, as well as trophoblast cells and normal blood lymphocytes and granulocytes. IDE protein was expressed in all the tissues assessed and all the tumor cell lines except for Raji and HL-60. Trophoblast cells and granulocytes, but not normal lymphocytes, were also IDE-positive.

Keywords: antibody; immunohistochemistry; insulinase; inverzincin; normal tissue; tumor cell line
Corresponding author
Received: 2008-4-21
Accepted: 2008-7-23
Published Online: 2008-09-11
Published in Print: 2008-11-01

©2008 by Walter de Gruyter Berlin New York

From the journal
Biological Chemistry
Biological Chemistry
Volume 389 Issue 11
Submit manuscript

Journal and Issue

Articles in the same Issue

Guest Editorial
Highlight: sphingolipids – signals and disease
Highlight: Sphingolipid Signaling
Sphingolipid management by an orchestra of lipid transfer proteins
Acid β-glucosidase: insights from structural analysis and relevance to Gaucher disease therapy
Ceramide in bacterial infections and cystic fibrosis
Relevance and potential of sphingosine-1-phosphate in vascular inflammatory disease
Nitric oxide and sphingolipids: mechanisms of interaction and role in cellular pathophysiology
Sphingosine kinase 1 and 2 regulate the capacity of mesangial cells to resist apoptotic stimuli in an opposing manner
Genes and Nucleic Acids
Regulation of human tissue kallikrein-related peptidase expression by steroid hormones in 32 cell lines
Protein Structure and Function
Relevance of the amino acid conversions L144R (Zaragoza) and L159P (Zavalla) in the apolipoprotein A-I binding site for haptoglobin
Molecular Medicine
Molecular profile of breast versus ovarian cancer cells in response to treatment with the anticancer drugs cisplatin, carboplatin, doxorubicin, etoposide and taxol
Cell Biology and Signaling
Regulation of kinin B2 receptors by bradykinin in human lung cells
Proteolysis
Immunohistochemical evidence of ubiquitous distribution of the metalloendoprotease insulin-degrading enzyme (IDE; insulysin) in human non-malignant tissues and tumor cell lines
Downloaded on 17.5.2024 from https://www.degruyter.com/document/doi/10.1515/BC.2008.157/html
Scroll to top button