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Licensed Unlicensed Requires Authentication Published by De Gruyter June 1, 2005

Trichostatin A Modulates Expression of p21waf1/cip1, Bcl-xL, ID1, ID2, ID3, CRAB2, GATA-2, hsp86 and TFIID/TAFII31 mRNA in Human Lung Adenocarcinoma Cells

  • Bodo Eickhoff , Stephan Rüller , Thomas Laue , Gotje Köhler , Christian Stahl , Max Schlaak and Jürgen van der Bosch
From the journal Biological Chemistry

Abstract

Lung adenocarcinoma cells treated for 16 h with trichostatin A (TSA), an inhibitor of histone deacetylases, and untreated cells were analyzed with respect to differential gene expression. Complex hybridization of cDNA arrays revealed repression of Bcl-xL, CRAB2 and TFIID/TAFII31 as well as induction of p21waf1/cip1, GATA-2, hsp86, ID1, ID2 and ID3 mRNA expression, which could be verified by Northern blotting. ID2 induction was further confirmed by Taqman realtime quantitative RT-PCR. The described alterations of gene expression due to TSA renders the lung adenocarcinoma cells susceptible to induction of apoptosis.

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Published Online: 2005-06-01
Published in Print: 2000-02-15

Copyright © 2000 by Walter de Gruyter GmbH & Co. KG

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