Kinesin-14-II is a plant-specific subclass of the kinesin-14 subfamily and includes kinesins with a calponin homology domain (KCHs). A tobacco member of kinesin-14-II, TBK11 is a viable candidate responsible for microtubule-dependent nuclear movement because of its apparent localization to the nuclear envelope. In tobacco BY-2 cells, it has been reported that the nuclear migration from the cell periphery to the central region in the pre-mitotic phase requires microtubules; however, the contribution of microtubules to the movement of daughter nuclei away from the cell plate in the post-cytokinetic phase has not been clarified. In the present study, we performed truncation analysis of TBK11 and found that TBK11 associates with the nuclear envelope via its central coiled-coil domain. We also found that the movement of daughter nuclei away from the cell plate is inhibited by treatment with microtubule inhibitors. These results suggest that TBK11 potentially plays a role in pre-mitotic and post-cytokinetic nuclear movement through interaction with the nuclear envelope via its central coiled-coil domain.