Karyotypes of five species of parasitic wasps of the superfamily Chalcidoidea, namely: Eurytoma flavimana Boheman (Eurytomidae; 2n=20), Perilampus rushkai Hellén (Perilampidae; 2n=6), Ormyrus gratiosus (Foerster) (Ormyridae; 2n=10), Megastigmus pictus (Foerster) (2n=10) and Megastigmus strobilobius Ratzeburg (Torymidae; n=5 and 2n=10) are studied for the first time, including first chromosome records for the family Perilampidae and subfamily Megastigminae (Torymidae). 2n=12 is confirmed in the previously studied Ormyrus sp. Phylogenetic and taxonomic implications of the new chromosomal data are discussed.