Folia Parasitologica 62[1] 26 (2015) | DOI: 10.14411/fp.2015.026

Morphological and attachment changes of Lamellodiscus theroni (Monogenea: Diplectanidae) during its post-larval development on fish

Neus Sánchez-García, Juan A. Raga, Francisco E. Montero
Cavanilles Institute of Biodiversity and Evolutionary Biology, Science Park, University of Valencia, Paterna, Spain

Species of the genus Lamellodiscus Johnston et Tiegs, 1922 (Monogenea: Diplectanidae) are characterised by a complex haptor bearing many different attachment elements: two pairs of main hooks joined by medial bars, 14 peripheral marginal hooks and one or two lamellodiscs, formed by several overlapping sclerotised plates (lamellae). These haptoral structures appear gradually during parasite development and, therefore, attachment strategies vary with developmental stage. The main aim of this work was to study the developmental changes of Lamellodiscus theroni Amine, Euzet et Kechemir-Issad, 2007 under experimental conditions, with special attention to the gradual variations in attachment strategies and the pathological implications. Throughout the gradual development of the sclerotised structures, six developmental phases were distinguished in L. theroni: phase I, with only 14 peripheral marginal hooks; phase II, with main hooks (ventral and dorsal) formed; phase III, with ventral bar formed; phase IV, with dorsal bars formed; phase V, with dorsal and ventral lamellodiscs formed; and phase VI, adult stage with male copulatory organ formed. During development, parasites attach to different parts of the first and secondary gill lamellae and the mode of attachment changes from unspecific stage, i.e. based on piercing any flat gill tissue in the early stages, through an intermediate stage when ventral and dorsal main hooks are completely functional and parasites become restricted to the interlamellar space, and finally to the definitive adult attachment stage when lamellodiscs are fully developed. The timing of key events in the development of L. theroni was used to establish adequate intervals for anthelmintic drug administration.

Keywords: anthelmintic, chronology, haptor growth, lamellodisc, sclerites

Received: June 3, 2014; Accepted: January 10, 2015; Published online: May 26, 2015  Show citation

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Sánchez-García, N., Raga, J.A., & Montero, F.E. (2015). Morphological and attachment changes of Lamellodiscus theroni (Monogenea: Diplectanidae) during its post-larval development on fish. Folia Parasitologica62, Article 2015.026. https://doi.org/10.14411/fp.2015.026
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References

  1. Amine F., Euzet L., Kechemir-Issad N. 2007: Lamellodiscus theroni sp. nov. (Monogenea, Diplectanidae), a gill parasite from Diplodus puntazzo (Teleostei, Sparidae) from the Mediterranean Sea. Acta Parasitol. 52: 305-309. Go to original source...
  2. Bychowsky B.E. 1957: Monogenetic Trematodes. Their Systematics and Phylogeny. English translation. American Institute of Biological Sciences, Washington, D.C., 1961, 627 pp.
  3. Cecchini S. 1994: Influence of temperature on the hatching of eggs of Diplectanum aequans, a parasite of sea bass. Aquacult. Int. 2: 249-253.
  4. Cecchini S., Saroglia M., Berni P., Cognetti-Varriale M. 1998: Influence of temperature on the life cycle of Diplectanum aequans (Monogenea, Diplectanidae), parasitic on sea bass, Dicentrarchus labrax (L.). J. Fish Dis. 21: 73-75. Go to original source...
  5. Desdevises Y. 2001: The phylogenetic position of Furnestinia echeneis (Monogenea, Diplectanidae) based on molecular data: a case of morphological adaptation? Int. J. Parasitol. 31: 205-208. Go to original source... Go to PubMed...
  6. Dezfuli B.S., Giari L., Simoni E., Menegatti R., Shinn A.P., Manera M. 2007: Gill histopathology of cultured European sea bass, Dicentrarchus labrax (L.), infected with Diplectanum aequans (Wagener 1857) Diesing 1958 (Diplectanidae: Monogenea). Parasitol. Res. 100: 707-713. Go to original source... Go to PubMed...
  7. Domingues M.V., Boeger W.A. 2008: Phylogeny and revision of Diplectanidae Monticelli, 1903 (Platyhelminthes: Monogenoidea). Zootaxa 1698: 1-40. Go to original source...
  8. Dzika E., Dzikowiec M., Hoffmann R.W. 2009: Description of the development of the attachment and copulatory apparatus of Dactylogyrus extensus from Cyprinus carpio var. koi. Helminthologia 46: 39-44. Go to original source...
  9. Euzet L. 1957: Recherches sur les Monogenoidea parasites de poissons marins. Ann. Parasitol. Hum. Comp. 32: 469-481. Go to PubMed...
  10. Euzet L., Audouin J. 1959: Sur un genre nouveau de Monogenoidea parasite de la dorade Chrysophrys aurata. L. Rev. Trav. Inst. Pêches Marit. 23: 317-322.
  11. Katharios P., Hayward C., Papandroulakis N., Divanach P. 2006: Pathology of Lamellodiscus spp. (Monogenea) parasitizing the gills of sharpsnout seabream and preliminary results of formalin treatment. Bull. Eur. Assoc. Fish Pathol. 26: 196-201.
  12. Kearn G.C. 1968: The development of the adhesive organs of some diplectanid, tetraonchid and dactylogyrid gill parasites (Monogenea). Parasitology 58: 149-163. Go to original source... Go to PubMed...
  13. Kearn G.C. 2004: Monogenean gill parasites, polyopisthocotyleans. In: G.C. Kearn (Ed.), Leeches, Lice and Lampreys. A Natural History of Skin and Gill Parasites of Fishes. Springer, Norwell, pp. 103-130.
  14. Mladineo I. 2006: Parasites of Adriatic cage reared fish. Acta Adriat. 47: 23-28.
  15. Mo T.A. 1993: Seasonal variations of the opisthaptoral hard parts of Gyrodactylus derjavini Mikailov, 1975 (Monogenea: Gyrodactylidae) on brown trout Salmo trutta L. parr and Atlantic salmon S. salar L. parr in the River Sandvikselva, Norway. Syst. Parasitol. 26: 225-231. Go to original source...
  16. Ogawa K. 1988: Development of Bivagina tai (Monogenea, Microcotylidae). Nippon Suisan Gakkaishi 54: 61-64. Go to original source...
  17. Oliver G. 1987: Les Diplectanidae Bychowsky 1957 (Monogenea, Monopisthocotylea, Dactylogyridea). Systématique, biologie, ontogénie, écologie, essai de phylogénèse. Thèse d'état, Université des Sciences et Techniques du Languedoc, Montpellier, 433 pp.
  18. Paling J.E. 1966. The attachment of the monogenean Diplectanum aequans (Wagener) Diesing to the gills of Morone labrax L. Parasitology 56: 493-503. Go to original source... Go to PubMed...
  19. Prost M. 1963: Investigations on the development and pathogenicity of Dactylogyrus anchoratus (Duj. 1845) and D. extensus Mueller et v. Cleave, 1932 for breeding carps. Acta Parasitol. Pol. 11: 17-47.
  20. Repullés-Albelda A., Holzer A.S., Raga J.A., Montero F.E. 2012: Oncomiracidial development, survival and swimming behaviour of the monogenean Sparicotyle chrysophrii (van Beneden and Hesse, 1863). Aquaculture 338-341: 47-55. Go to original source... Go to PubMed...
  21. Repullés-Albelda A., Raga J.A., Montero F.E. 2011: Post-larval development of the microcotylid monogenean Sparicotyle chrysophrii (van Beneden and Hesse, 1863): comparison with species of Microcotylidae and Heteraxinidae. Parasitol. Int. 60: 512-520. Go to original source... Go to PubMed...
  22. Roubal F.R., Diggles B.K. 1993: The rate of development of Polylabroides multispinosus (Monogenea: Microcotylidae) parasitic on the gills of Acanthopagrus australis (Pisces: Sparidae). Int. J. Parasitol. 23: 871-875. Go to original source... Go to PubMed...
  23. Rubio-Godoy M., Tinsley R.C. 2002: Trickle and single infection with Discocotyle sagittata (Monogenea: Polyopisthocotylea): effect of exposure mode on parasite abundance and development. Folia Parasitol. 49: 269-278. Go to original source... Go to PubMed...
  24. Sánchez-García N., Ahuir-Baraja A.E., Raga J.A., Montero F.E. 2013: Morphometric, molecular and ecological analyses of the parasites of the sharpsnout seabream Diplodus puntazzo Cetti (Sparidae) from the Spanish Mediterranean: implications for aquaculture. J. Helmintol. 3: 1-15. Go to original source...
  25. Sánchez-García N., Padrós F., Raga J.A., Montero F.E. 2011: Comparative study of the three attachment mechanisms of diplectanid monogeneans. Aquaculture 318: 290-299. Go to original source...
  26. Toksen E. 2006: Occurrence of gill parasite Lamellodiscus ignoratus (Palombi, 1943) (Monogenea: Diplectanidae) in cultured sharp-snout sea bream, Diplodus puntazzo (Cetti, 1777) from Izmir, Turkey. Bull. Eur. Assoc. Fish Pathol. 26: 174-179.
  27. Whittington I.D., Chisholm L.A. 2008: Diseases caused by Monogenea. In: J.C. Eiras, H.S.T. Wahlii and B.G. Kapoor (Eds.), Fish Diseases. Science Publishers, New Hampshire, pp. 683-816.