Cloning the sterol carrier protein 2 genes of Japanese toad（Bufo japonicus formosus） and Chinese toad（Bufo gargarizans） and its tissue expression analysisEnglish Full Text

Yu-Cheng JI;Hui ZHUGE;Shan-Shan ZHANG;Shu-Fang ZHANG;Xian-Yu Yang;School of Forestry and Biotechnology,Zhejiang Agricultural and Forestry University;The Nurturing Station for the State Key Laboratory of Subtropical Silviculture,Zhejiang Agricultural and Forestry University;College of Animal Science and Technology,Zhejiang Agricultural and Forestry University;

Abstract: In this study,to clarify the bioactive polypeptides included in the skins and secretions of Bufo,we screened the Japanese toad（Bufo japonicus formosus） skin cDNA library by colony polymerase chain reaction（PCR）,and obtained a transcript of 1 075 bp consisting of 1 37 bp 5′ untranslated region（UTR）,515 bp 3′ UTR and a 423 bp open reading frame（ORF） encoding a polypeptide of 140 amino acid residues（GenBank accession number: KF359945）.Homolog analysis showed a 70%–96% homology with sterol carrier protein-2（SCP-2） present in other animals,which is implicated in lipid metabolism of other organisms.The gene SCP-2 of Chinese toad（B.gargarizans） was cloned from a first strand cDNA of Bufo skin（GenBank accession number: KF381341） via PCR,whose encoding polypeptide has only one amino acid difference from that of Japanese toad.Tissue distribution analysis showed that SCP-2 expressed in all organs tested,though in the liver and spleen it manifested lower expression than in other organs.These findings might indicate SCP-2 being one of the active ingredients in toad skin.These findings may in turn have implications for further drug development from traditional Chinese medicine sources.

Keywords：

Bufo gargarizans; Bufo japonicus formosus; SCP-2 cDNA cloning; Tissue expression;

DOI：
10.13918/j.issn.2095-8137.2014.5.398
Series：
Subject：
Classification Code：
Q78

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Cloning the sterol carrier protein 2 genes of Japanese toad（Bufo japonicus formosus） and Chinese toad（Bufo gargarizans） and its tissue expression analysisEnglish Full Text

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