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Cloning genes from a library using a clustering strategy and PCR

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Abstract

A new polymerase chain reaction (PCR)-based method is described for the isolation of clones of interest from a library when only part of a sequence is available. In actuality, this occurs with many genomes that have been partially sequenced using a random strategy. The method presented here, discriminating clusters by PCR (DCbyPCR), is a nonradioactive and improved alternative to colony hybridization.

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Authors and Affiliations

  1. Department of Molecular and Cell Biology, University of A Coruña, Campus da Zapateira s/n, 15071-A, Coruña, Spain

    S. Díaz Prado, N. Tarrío, M. E. Cerdán & M. I. González Siso

Authors
  1. S. Díaz Prado
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  2. N. Tarrío
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  3. M. E. Cerdán
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  4. M. I. González Siso
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Corresponding author

Correspondence to M. I. González Siso.

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Díaz Prado, S., Tarrío, N., Cerdán, M.E. et al. Cloning genes from a library using a clustering strategy and PCR. Mol Biotechnol 26, 35–38 (2004). https://doi.org/10.1385/MB:26:1:35

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  • DOI: https://doi.org/10.1385/MB:26:1:35

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