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Thyrotropin-releasing hormone increases phospholipase D activity through stimulation of protein kinase C in GH3 cells

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Abstract

Activation of phospholipase D was investigated after treatment of GH3 cells with thyrotropin-releasing hormone. Thyrotropin-releasing hormone treatment resulted in both time- and dose-dependent increases of phospholipase D activity, translocation of protein kinase C-α and -βl isozymes from cytosol to membrane within 30 min, and approx 43-fold increase of phosphatidylinositol-specific phospholipase C activity. Intracellular calcium concentration was rapidly increased and diacyglycerol level remained high up to 3 h after the treatment. Pretreatment of the cells with U73122, a potent inhibitor of phosphatidylinositol-specific phospholipase C, inhibited thyrotropin-releasing hormone-induced phospholipase D activation. Protein kinase C activity was down-regulated by pretreatment of the GH3 cells with either protein kinase C inhibitors (RO320432, GF109203X) or preincubation of the cells with phorbol myristrate acetate (500 nM) for 24 h. This treatment largely abolished the thyrotropin-releasing hormone-induced activation of phospholipase D, thus further confirming the involvement of protein kinase C in the activation. These results suggest that thyrotropin-releasing hormone-induced phospholipase D activation may be due to phosphatidylinositol-specific phospholipase C, and activation of protein kinase C isozymes is responsible for this stimulation.

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Authors and Affiliations

  1. Department of Internal Medicine, College of Medicine, Hanyang University, 133-791, Seoul, Korea

    Dong-Sun Kim & Tae-Wha Kim

  2. Institute of Biomedical Science and Department of Biochemistry, College of Medicine, Hanyang University, 17 Haengdang-Dong, Sungdon-Gu, 133-791, Seoul, Korea

    Mee-Sup Yoon & Joong-Soo Han

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  1. Dong-Sun Kim
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  2. Mee-Sup Yoon
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  3. Tae-Wha Kim
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  4. Joong-Soo Han
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Correspondence to Joong-Soo Han.

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Kim, DS., Yoon, MS., Kim, TW. et al. Thyrotropin-releasing hormone increases phospholipase D activity through stimulation of protein kinase C in GH3 cells. Endocr 23, 33–38 (2004). https://doi.org/10.1385/ENDO:23:1:33

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  • DOI: https://doi.org/10.1385/ENDO:23:1:33

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