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c-Jun targets amino terminus of androgen receptor in regulating androgen-responsive transcription

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Abstract

The human androgen receptor (hAR) is a member of the nuclear receptor superfamily and functions as a ligand-inducible transcription factor. We have previously proposed that c-Jun mediates the transcriptional activity of this receptor. The modular nature of hAR was used in this study to generate several fusions with the heterologous DNA-binding domain of the yeast transcription factor GAL4 in an attempt to identify the c-Jun-responsive domains within the receptor. Our results suggest that the target of c-Jun action is the amino terminus (AB region) of the receptor and that hAR amino acids 502–521 are critical for the c-Jun response. Additionally, amino acids 503–555 were shown to harbor an autonomous transactivation that is stimulated by c-Jun. Furthermore, we demonstrated that transcription intermediary factor-2 (TIF-2), a coactivator that acts on the activation function-2, stimulates the full-length hAR. These results suggest that c-Jun and TIF-2 can work together as coactivators on the hAR by targeting distinct portions of the receptor.

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Authors and Affiliations

  1. Cold Spring Harbor Labs, 1 Bungtown Road, Cold Spring Harbor, NY

    Athanasios Bubulya

  2. Department of Cell Biology, Parke-Davis Pharmaceutical Research, Ann Arbor, MI

    Xi-Qiang Shen

  3. Department of Biology, University of Toledo, 43606, Toledo, OH

    Athanasios Bubulya, Xiao-Feng Zhou, Xi-Qiang Shen, Christopher J. Fisher &  Lirim Shemshedini

Authors
  1. Athanasios Bubulya
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  2. Xiao-Feng Zhou
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  3. Xi-Qiang Shen
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  4. Christopher J. Fisher
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  5. Lirim Shemshedini
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Correspondence to Lirim Shemshedini.

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Bubulya, A., Zhou, XF., Shen, XQ. et al. c-Jun targets amino terminus of androgen receptor in regulating androgen-responsive transcription. Endocr 13, 55–62 (2000). https://doi.org/10.1385/ENDO:13:1:55

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  • DOI: https://doi.org/10.1385/ENDO:13:1:55

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