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Preparation of proteolytic enzymes from kamchatka crab Paralithodes camchatica hepatopancreas and their application

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Abstract

A method was elaborated to obtain “Moricrase”, a complex of collageno and fibrinolytic proteinases from Paralithodes camchatica hepatopancreas. Moricrol, a Moricrase-containing ointment, was shown to exhibit wound-healing activity in the treatment of burns and trophic ulceration as well as postoperative scars. The following homogeneous proteinases were isolated from Moricrase: collagenolytic serine proteinase, trypsin, metalloproteinase, carboxypeptidase, and aminopeptidase. An efficient collagenolytic activity of Moricrase is a result of cooperative action of these enzymes.

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References

  1. Klimova, O. A., Borukhov, S. I., Solovyeva, N. I., Balaevskaya, T. O., and Strongin, A. Ya. (1990), Biochem. Biophys. Res. Commun., 166, 1411–1420.

    Article  CAS  Google Scholar 

  2. Sakharov, I. Yu., Litvin, F. E., and Artyukov, A. A. (1992), Biokhimia (Russian) 57, 40–50.

    CAS  Google Scholar 

  3. Saharov, I. Yu., and Dzhunkovskaya, A. V. (1993), Biokhimia (Russian) 58, 1145–1153.

    Google Scholar 

  4. Klimova, O. A., Vedishcheva, Yu. V., and Strongin, A. Ya. (1991), Dokl. Akad. Nauk SSSR (Russian) 317, 482–484.

    CAS  Google Scholar 

  5. Grant, G. A. and Eisen, A. Z. (1980), Biochemistry 19, 6089–6095.

    Article  CAS  Google Scholar 

  6. Grant, G. A., Sacchettini, J. C., and Welgus, H. G. (1983), Biochemistry 22, 354–358.

    Article  CAS  Google Scholar 

  7. Filippova, I. Yu., Lysogorskaya, E. N., Oksenoit, E. S., Rudenskaya, G. N., and Stepanov, V. M. (1984), Analyt. Biochem., 143, 293–297.

    Article  CAS  Google Scholar 

  8. Shaginyan, K. A., Izotova, L. S., Iomantas, Yu. V., Strongin, A. Ya., and Stepanov, V. M. (1980), Biokhimia (Russian) 45, 2083–2095.

    CAS  Google Scholar 

  9. Astrup, T. and Mullerz, S. (1952), Arch. Biochem. Biophys. 40, 345–351.

    Article  Google Scholar 

  10. Neuman, R. E. and Zogan, M. A. (1950), J. Biol. Chem. 184, 299–306.

    CAS  Google Scholar 

  11. Causton, T. E. and Murphy, G. (1981), Meth. Enzymol. 80, 711–722.

    Article  Google Scholar 

  12. Rudenskaya, G. N., Isaev, V. A., Stepanov, V. M., Dunaevsky, Ya. E., Baratova, L. A., Kalebina, T. S., and Nurminskaya, M. V. (1996), Biochemistry Moscow 61, 804–814.

    Google Scholar 

  13. Kulaev, I. S., Solovyeva, N. I., Kalebina, T. S., Rudenskaya, G. N., and Nurminskaya, M. V. (1988), Dokl. Acad. Nauk SSSR (Russian) 303, 499–503.

    CAS  Google Scholar 

  14. Rudenskaya, G. N., Isaev, V. A., Kalebina, T. S., Stepanov, V. M., Mal'tsev, K. V., Shvets, S. V., Luk'anova, N. A., Kislitsin, Yu. A., and Miroshnikov, A. I. (1998), Biorg. Khim. (Russian) 24, 162–118.

    Google Scholar 

  15. Stocker, W. (1992), Nature 358, 9–14.

    Google Scholar 

  16. Rudenskaya, G. N., Kupenko, O. G., Isaev, V. A., Stepanov, V. M., and Dunaevsky, Ya. E. (1995), Biorg. Khim. (Russian) 21, 249–255.

    CAS  Google Scholar 

  17. Isaev, V. A., Karabasova, M. A., Lutova, L. V., Rudenskaya, G. N., Kupenko, O. G., and Stepanov, V. M. (1992), Russian patent N2057534,8,6.

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Authors and Affiliations

  1. School of Chemistry, Moscow State University, 119899, Moscow, Russia

    G. N. Rudenskaya, V. A. Isaev, A. M. Shmoylov & M. A. Karabasova

  2. M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 119840, GSP-3, Moscow, Russia

    S. V. Shvets & A. I. Miroshnikov

  3. CNII of Stomatology, 119840, GSP-3, Moscow, Russia

    A. B. Brusov

Authors
  1. G. N. Rudenskaya
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  2. V. A. Isaev
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  3. A. M. Shmoylov
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  4. M. A. Karabasova
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  5. S. V. Shvets
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  6. A. I. Miroshnikov
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  7. A. B. Brusov
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Rudenskaya, G.N., Isaev, V.A., Shmoylov, A.M. et al. Preparation of proteolytic enzymes from kamchatka crab Paralithodes camchatica hepatopancreas and their application. Appl Biochem Biotechnol 88, 175–183 (2000). https://doi.org/10.1385/ABAB:88:1-3:175

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  • DOI: https://doi.org/10.1385/ABAB:88:1-3:175

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