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Solid-state fermentation with Aspergillus niger for cellobiase production

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Abstract

Aspergillus niger NRRL3 was cultivated in a moist wheat bran and ground corncob solid medium supplemented with inorganic minerals for the production of cellobiase (β-1,4-glucosidase, EC 3.2.1.21). With this method, A. niger NRRL3 was able to produce a high concentration of cellobiase (215 IU/gofsolid substrate) after 96 h of incubation. Temperature and moisture content affected final cellobiase titers. The best conditions for cell obiase production from solid substrate by A. niger NRRL3 were determined to be 70% moisture and 35°C.

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Authors and Affiliations

  1. Laboratory of Renewable Resources Engineering, Purdue University, 47907, West Lafayette, IN

    George T. Tsao, Liming Xia, Ningjun Cao & Cheng S. Gong

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  1. George T. Tsao
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  2. Liming Xia
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  3. Ningjun Cao
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  4. Cheng S. Gong
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Tsao, G.T., Xia, L., Cao, N. et al. Solid-state fermentation with Aspergillus niger for cellobiase production. Appl Biochem Biotechnol 84, 743–749 (2000). https://doi.org/10.1385/ABAB:84-86:1-9:743

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  • DOI: https://doi.org/10.1385/ABAB:84-86:1-9:743

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