Abstract
Pretreatment has been recognized as a key step in enzyme-based conversion processes of lignocellulose biomass to ethanol. The aim of this study is to evaluate two hydrothermal pretreatments (steam explosion and liquid hot water) to enhance ethanol production from poplar (Populus nigra) biomass by a simultaneous saccharification and fermentation (SSF) process. The composition of liquid and solid fractions obtained after pretreatment, enzymatic digestibility, and ethanol production of poplar biomass pretreated at different experimental conditions was analyzed. The best results were obtained in steam explosion pretreatment at 210°C and 4 min, taking into account cellulose recovery above 95%, enzymatic hydrolysis yield of about 60%, SSF yield of 60% of theoretical, and 41% xylose recovery in the liquid fraction. Large particles can be used for poplar biomass in both pretreatments, since no significant effect of particle size on enzymatic hydrolysis and SSF was obtained.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
El Bassam, N. (1996), in Renewable Energy: Potential Energy Crops for Europe and the Mediterranean Region, FAO, Rome, Italy, Rev. Technical Series 46, 142–156.
Sun, Y. and Cheng, J. (2002), Bioresour. Technol. 83, 1–11.
McMillan, J. D. (1994) in Enzymatic Conversion of Biomass for Fuels Production, Himmel, M. E., Baker, J. O., and Overend, R. P., eds., American Chemical Society, Washington, DC, pp. 292–324.
Heitz, M., Capek-Ménard, E., Koeberle, P. G., Gagné, J., and Chornet, E. (1991), Bioresour. Technol. 35, 23–32.
Mes-Hartree, M. and Saddler, J. N. (1983), Biotechnol. Lett. 5, 531–536.
Ando, S., Arai, I., Kiyoto, K., and Hanai, S. (1986), J. Ferment. Technol. 64, 567–570.
Duff, S. J. B. and Murray, W. D. (1996), Bioresour. Technol. 55, 1–33.
Wright, J. D. (1998), Chem. Eng. Prog. 84, 62–74.
Van Walsum, G. P., Allen, S. G., Spenser, M. J., Laser, M. S., Antal, M. J., and Lynd, L.R. (1996), Appl. Biochem. Biotechnol. 57–58, 157–170.
Weil, J., Sarikaya, A., Rau, S. L., Goetz, J., Ladisch, C. M., Brewer, M., Hendrickson, R., and Ladisch, M. R. (1997), Appl. Biochem. Biotechnol. 68, 21–40.
Weil, J. P., Sarikaya, A., Rau, S. L., Goetz, J., Ladish, M., Brewer, M., and Hendrickson, R. (1998), Appl. Biochem. Biotechnol. 73, 1–17.
Mok, W. S.-L. and Antal, M. J. (1992), Ind. Eng. Chem. Res. 31, 1157–1161.
Laser, M., Schulman D., Allen, S. G., Lichwa J., Antal, M. J., and Lynd, L. R. (2002), Bioresour. Technol. 81, 33–44.
Allen, S. G., Schulman D., Lichwa, J., and Antal, M.J. (2001), Ind. Eng. Chem. Res. 40, 2934–2941.
Ulbricht, R. J., Northum, S. J., and Thomas, J. A. (1984), Fund. Appl. Toxicol. 4, 843–853.
Carrasco, J. E., Martinez, J. M., Negro, M. J., Manero, J., Mazón, P., Sáez, F., and Martín, C. (1989), in Biomass for Energy and Industry, 5th Conference, vol. 2, Grassi, G., Gosse, G., and Dos Santos, G., eds., Elsevier, Essex, England, UK, pp. 38–44.
Ballesteros, I., Oliva, J. M., Navarro, A. A., González, A., Carrasco, J., and Ballesteros, M. (2000), Appl. Biochem. Biotechnol. 84–86, 97–110.
Ballesteros, I., Oliva, J. M., Negro, M. J., Manzanares, P., and Ballesteros, M. (2002), Appl. Biochem. Biotechnol. 98–100, 717–732.
Ballesteros, I., Ballesteros, M., Cabañas, A., Carrasco, J., Martín, C., Negro, M. J., Sáez, F., and Sáez, R. (1991), Appl. Biochem. Biotechnol. 28–29, 307–315.
Ruiz, R. and Ehrman, T. (1996), NREL Chemical Analysis and Testing Laboratory Analytical Procedure, No. 002., National Renewable Energy Laboratory, Golden, CO.
Templeton, D. and Ehrman, T. (1995), NREL Chemical Analysis and Testing Laboratory Analytical Procedure, No. 003., National Renewable Energy Laboratory, Golden, CO.
Weil, J., Brewer, M., Hendrickson, R., Sarikaya, A., and Ladish, M. (1998), Appl. Biochem. Biotechnol. 70–72, 99–111.
Belkacemi, K.; Abatzoglou, N., Overed, R. P., and Chornet, E. (1991), Ind. Eng. Chem. Res. 30, 2416–2425.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Negro, M.J., Manzanares, P., Ballesteros, I. et al. Hydrothermal pretreatment conditions to enhance ethanol production from poplar biomass. Appl Biochem Biotechnol 105, 87–100 (2003). https://doi.org/10.1385/ABAB:105:1-3:87
Issue Date:
DOI: https://doi.org/10.1385/ABAB:105:1-3:87