Abstract
Banana bunchy top disease is a major constraint to banana production in most regions where this crop is grown. The disease is caused by Banana bunchy top virus (BBTV), a multicomponent, single-stranded DNA virus of the family Nanoviridae. We have designed primers to a conserved region of the master replication-associated protein that are useful for the polymerase chain reaction (PCR)-mediated detection of BBTV. In addition, primers to banana genomic sequence are used as an internal control, overcoming the uncertainty (owing to false-negatives) inherent in PCR diagnostics. Together these primer sets are a valuable tool in the effort to control BBTV, particularly in screening micropropagated banana plantlets for the absence of virus before release to farmers.
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Mansoor, S., Qazi, J., Amin, I. et al. A PCR-based method, with internal control, for the detection of Banana bunchy top virus in banana. Mol Biotechnol 30, 167–169 (2005). https://doi.org/10.1385/MB:30:2:167
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DOI: https://doi.org/10.1385/MB:30:2:167