Abstract
Methods to microencapsulate enzyme, cells, and genetically engineered cells have been described in this article. More specific examples of enzyme encapsulation include the microencapsulation of xanthine oxidase for Lesch-Nyhan disease; phenylalanine ammonia lyase for pheny, ketonuria and microencapsulation of multienzyme systems with cofactor recycling for multistep enzyme conversions. Methods for cell encapsulation include the details for encapsulating hepatocytes for liver failure and for gene therapy. This also includes the details of a novel two-step method for encapsulation of high concentrations of smaller cells. Another new approach is the detailed method of the encapsulation of genetically engineered Escherichia coli DH5 cells for lowering urea, ammonia, and other metabolites in kidney or, liver failure and other diseases.
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Chang, T. M. S. (1964) Semipermeable microcapsules. Science 146, 524–525.
Chang, T. M. S., MacIntosh, F. C., and Mason, S. G. (1966) Semipermeable aqueous microcapsules: I. Preparation and properties. Can. J. Physiol. Pharmacol., 44, 115–128.
Chang, T. M. S., MacIntosh, F. C., and Mason, S. G. (1971) Encapsulated hydrophilic compositions and methods of making them. Canadian Patent, 873, 815, 1971.
Chang, T. M. S. (1972) “Artificial Cells” Monograph, Charles C Thomas Publisher, Springfield, U.S.A. 1972.
Chang, T. M. S. (1995) Artificial cells with emphasis on bioencapsulation in biotechnology. Biotechnology Annual Review 2, 267–295.
Chang, T. M. S. (1997) Recent and future developments in modified hemoglobin and microencapsulated hemoglobin as red blood cell substitutes. Artificial Cells, Blood Substitutes & Immobilization Biotechnology 25, 1–24.
Chang TMS (1997) “Blood Substitutes: principles, methods, products and clinical trials.” Karger/Landes Co. Austin, Texas.
Chang, T. M. S. and W.P. Yu (1996) Biodegradable polymer membrane containing hemoglobin for blood substitutes. U.S.A. Patent approved in 1996.
Yu, W. P. and Chang, T. M. S. (1996) Submicron polymer membrane hemoglobin nanocapsules as potential blood substitutes: preparation and characterization. Artificial Cells, Blood Substitutes & Immobilization Biotechnology, an international journal 24, 169–184.
Chang, T.M.S. and Poznansky, M. J. (1968) Semipermeable microcapsules containing catalase for enzyme replacement in acatalsaemic mice. Nature 218(5138), 242–245.
Chang, T. M. S. (1971) The in vivo effects of semipermeable microcapsules containing L-asparaginase on 6C3HED lymphosarcoma. Nature 229(528):117–118.
Chang, T. M. S. (1989) Preparation and characterization of xanthine oxidase immobilized by microencapsulation in artificial cells for the removal of hypoxanthine. J. Biomat., Artif. Cells. and Artif. Organs 17, 611–166.
Palmour, R. M., Goodyer, P., Reade, T., Chang, T. M. S. (1989) Microencapsulated xanthine oxidase as experimental therapy in Lesch-Nyhan Disease. Lancet 2(8664):687–688.
Chang, T. M. S., Bourget L., and Lister, C. (1992) Orgal administration of microcapsules for removal of amino acids, US Patent No 5,147,641.
Chang, T. M. S., Bourget, L., and Lister, C. (1995) A new theory of enterorecirculation of amino acids and its use for depleting unwanted amino acids using oral enzyme-artificial cells, as in removing phenylalanine in phenylketonuria. Artificial Cells, Blood Substitutes & Immobilization Biotechnology, 25, 1–23.303.
Bourget, L. and Chang, T. M. S. (1986) Phenylalanine ammonia-lyase immobilized in microcapsules for the depletion of phenylalanine in plasma in phenylketonuric rat model. Biochim. Biophys. Acta. 883:432–438.
Safos, S. and Chang, T. M. S. (1995) Enzyme replacement therapy in ENU2 phenylketonuric mice using oral microencapsulated phenylalanine ammonia-lyase: a preliminary report. Artificial Cells, Blood Substitutes & Immobilization Biotechnology, 25, 681–692.
Chang, T. M. S. (1985) Artificial cells with regenerating multienzyme systems. Methods in Enzymology 112, 195–203.
Gu, K. F. and Chang, T. M. S. (1990) Production of essential L-branched-chained amino acids, in bioreactors containing artificial cells immobilized multienzyme systems and dextran-NAD+. Applied Biochemistry and Biotechnology 26, 263–269.
Chang, T. M. S. (1965) Semipermeable Aqueous Microcapsules. Ph.D. Thesis. McGill University.
Lim, F. and Sun, A. M. (1980) Microencapsulated islets as bioartificial endocrine pancreas. Science 210, 908–909.
Wong, H. and Chang, T. M. S. (1986) Bioartificial liver: implanted artificial cells microencapsulated living hepatocytes increases survival of liver failure rats. Int. J. Artif. Organs 9, 335–336.
Wong, H. and Chang, T. M. S. (1998) The viability and regeneration of artificial cell microencapsulated rat hepatocyte xenograft transplants in mice. J. Biomat Artif. Cells and Artif. Organs 16, 731–740.
Kashani, S. and Chang, T. M. S. (1991) Effects of hepatic stimulatory factor released from free or microencapsulated hepatocytes on galactosamine induced fulminant hepatic failure animal model. J. Biomaterials, Artificial Cells and Immobilization Biotechnology 19, 579–598.
Bruni, S. and Chang, T. M. S. (1989) Hepatocytes immobilized by microencapsulation in artificial cells: Effects on hyperbilirubinemia in Gunn Rats. J Biomat Artif Cells and Artif Organs 17, 403–412.
Bruni, S. and Chang, T. M. S. (1991) Encapsulated hepatocytes for controlling hyper-bilirubinemia in Gunn Rats. Int. J. Artificial Organs 14, 239–241.
Bruni, S. and Chang, T. M. S. (1995) Kinetics of UDP-glucuronosyl-transferase in bilirubin conjugation by encnapsulated hepatocytes for transplantation into Gunn rats J. Artificial Organs 19, 449–457.
Wong, H. and Chang T. M. S. (1991) A novel two step procedure for immobilizing living cells in microcapsules for improving xenograft survival. Biomaterials, Artificial Cells and Immobilizing Biotechnology 19, 687–698.
Chang, T. M. S. and Wong, H. (1992) A novel method for cell encapsulation in artificial cells. USA Patent No. 5,084,350.
Prakash, S. and Chang, T. M. S. (1995) Preparation and in-vitro analysis of genetically engineered E. coli DH5 cells, microencapsulated in artificial cells for urea and ammonia removal. Biotechnology and Bioengineering, 46, 621–626.
Prakash, S. and Chang, T. M. S. (1996) Microencapsulated genetically engineered live E coli DH5 cells administered orally to maintain normal plasma urea level in uremic rats Nature Medicine 2, 883–887.
Friedman, E. A. (1999) Predicting nephrology in the 21st century. ASAIO J Sep–Oct;45(5):363–366.
Friedman, E. A. (1996) Bowel as a kidney substitute in renal failure. Am. J. Kidney Dis. 1996 Dec; 28(6):943–950.
Prakash, S. and Chang, T. M. S. (1999) Artificial cell microcapsules containing genetically engineered E. coli DH5 cells for in-vitro lowering of plasma potassium, phosphate, magnesium, sodium, chloride, uric acid, cholesterol, and creatinine: a preliminary report. Artif. Cells Blood Substit. Immobil. Biotechnol. Sep–Nov;27(5–6): 475–481.
Koo, J. and Chang, T. M. S. (1993) Secretion of erythropoietin from microencapsulated rat kidney cells: preliminary results. Int. J. Artif. Organs 16, 557–560.
Garofalo, F. and Chang, T. M. S. (1991) Effects of mass transfer and reaction kinetics on serum cholesterol depletion rates of free and immobilized Pseudomonas pictorum. Applied Biochemistry and Biotechnology 27, 75–91.
Lyold-George, I. and Chang, T. M. S. (1995) Characterization of free and alginate-polylysine-alginate microencapsulated Erwinia herbicola for the conversion of ammonia, pyruvate and phenol into L-tyrosine and L-DOPA J. Bioengineering and Biotechnology 48, 706–714.
Yu, Y. T. and Chang, T. M. S. (1981) Lipid-polymer membrane artificial cells containing multienzyme systems, cofactors and substrates for the removal of ammonia and urea. Trans. Am. Soc. Artif. Intern. Organs 27, 535–538.
Chang, T. M. S. (1971) Stabilization of enzyme by microencapsulation with a concentrated solution o or by crosslinking with glutaraldehyde. Biochem. Biophys. Res. Com. 44, 1531–1533.
Coromili, V. and Chang, T. M. S. (1993) Polydisperse dextran as a diffusing test solute to study the membrane permeability of alginate polylysine microcapsules. J. Biomaterials, Artificial Cells & Immobilization Biotechnology, 21, 323–335.
Chang, T. M. S. and Prakash, S. (1998) Therapeutic uses of microencapsulated genetically engineered cells. Molecular Medicine Today May; 4(5):221–227.
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Chang, T.M.S., Prakash, S. Procedures for microencapsulation of enzymes, cells and genetically engineered microorganisms. Mol Biotechnol 17, 249–260 (2001). https://doi.org/10.1385/MB:17:3:249
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DOI: https://doi.org/10.1385/MB:17:3:249