Abstract
The present studies were designed to determine how disrupting cell contact induces rat ovarian surface epithelial cells (i.e., ROSE-179 cells) to undergo apoptosis. In the first series of studies, the effect of depleting serum and calcium on the levels of the adhesion proteins N-cadherin and β-catenin was examined. These studies revealed that the depletion of serum and calcium results in the degradation of N-cadherin but not β-catenin. However, the localization of β-catenin changed from principally the plasma membrane to the nucleus. The nuclear localization of β-catenin was demonstrated by Western blot and confocal microscopy. A second series of studies demonstrated that cells that lost contact in response to the depletion of serum and calcium showed enhanced β-catenin-dependent transcription. Finally, forced expression of a stable form of β-catenin resulted in an increase in β-catenin within the cytoplasm of transfected ROSE-179 cells. When these β-catenin transfected ROSE-179 cells were deprived of serum and calcium, β-catenin accumulated within the nucleus and accelerated the rate at which these cells became apoptotic. These data indicate that in viable cells, β-catenin is part of the adhesion complex that maintains cell contact. If calcium-dependent cell contacts are broken, β-catenin accumulates within the nucleus, where it promotes transcription and ultimately the apoptotic death of ROSE-179 cells.
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Peluso, J.J., Pappalardo, A. & Hess, S.A. Effect of disrupting cell contact on the nuclear accumulation of β-catenin and subsequent apoptosis of rat ovarian surface epithelial cells in vitro. Endocr 12, 295–302 (2000). https://doi.org/10.1385/ENDO:12:3:295
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DOI: https://doi.org/10.1385/ENDO:12:3:295