Abstract
The aim of this study was to quantify the glucose modulation of the plasma membrane calcium pump (PMCA) function in rat pancreatic islets. Ca2+-ATPase activity and levels of phosphorylated PMCA intermediates both transiently declined to a minimum in response to stimulation by glucose. Strictly dependent on Ca2+ concentration, this inhibitory effect was fully expressed at physiological concentrations of the cation (less than 0.5 μM), then progressively diminished at higher concentrations. These results, together with those previously reported on the effects of insulin secretagogues and blockers on the activity, expression and cellular distribution of the PMCA, support the concept that the PMCA plays a key role in the regulation of Ca2+ signaling and insulin secretion in pancreatic islets.
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References
Wollheim, C. B. and Sharp, G. W. (1981) Regulation of insulin release by calcium. Physiol. Rev. 61, 914–973.
Hellman, B. (1986) Calcium transport in pancreatic beta-cells: implications for glucose regulation of insulin release. Diabetes Metab. Rev. 2, 215–241.
Carafoli, E. (1991) Calcium pump of the plasma membrane. Physiol. Rev. 71, 129–153.
Sagara, Y., Fernández-Belda, F., de Meis, L., and Inesi, G. (1992) Characterization of the inhibition of intracellular Ca2+ transport ATPases by thapsigargin. J. Biol. Chem. 267, 12606–12613.
Váradi, A., Molnár, E., and Ashcroft, S. J. H. (1995) Characterisation of endoplasmic reticulum and plasma membrane Ca2+-ATPases in pancreatic B-cells in islets of Langerhans. Biochim. Biophys. Acta 1236, 119–127.
Hoenig, M., Lee, R. J., and Ferguson, D. C. (1990) Glucose inhibits the high-affinity (Ca2++Mg2+)-ATPase in the plasma membrane of a glucose-responsive insulinoma. Biochim. Biophys. Acta 1022, 333–338.
Kamagate, A., Herchuelz, A., Bollen, A., and Van Eylen, F. (2000) Expression of multiple plasma membrane Ca2+-ATPases in rat pancreatic islet cells. Cell Calcium 27, 231–246.
Rossi, J. P., Gronda, C. M., Fernández, H. N., and Gagliardino, J. J. (1988) Characteristics of a Ca2+-ATPase activity measured in islet homogenates. Biochim. Biophys. Acta 943, 175–182.
Gronda, C. M., Rossi, J. P., and Gagliardino, J. J. (1988) Effect of different insulin secretagogues and blocking agents on islet cell Ca2+-ATPase activity. Biochim. Biophys. Acta 943, 183–189.
Gietzen, K., Wuethrich, A., and Bader, H. (1981) R 24571: a new powerful inhibitor of red blood cell Ca++-transport ATPase and of calmodulin-regulated functions. Biochim. Biophys. Res. Commun. 2, 418–425.
Pande, J., Mallhi, K. K., Sawh, A., Szewczyk, M. M., Simpson, F., and Grover, A. K. (2006) Aortic smooth muscle and endothelial plasma membrane Ca2+ pump isoforms are inhibited differently by the extracellular inhibitor caloxin 1b1. Am. J. Physiol. Cell Physiol. 290, C1341-C1349.
Gagliardino, J. J., and Rossi, J. P. F. C. (1994) Ca2+-ATPase in pancreatic islets: its possible role in the regulation of insulin secretion. Diabetes Metab. Rev. 10, 1–17.
Lacy, P. E. and Kostianovsky, M. (1967) Method for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes 16, 35–39.
García, M. E., del Zotto, H., Caride, A. J., Penniston, J. T., Rossi, J. P. F. C., and Gagliardino, J. J. (2002) Expression and cellular distribution pattern of plasma membrane calcium pump isoforms in rat pancreatic islets. J. Membr. Biol. 185, 17–23.
Echarte, M. M., Levi, V., Villamil, A. M., Rossi, R. C., and Rossi, J. P. F. C. (2001) Quantitation of plasma membrane calcium pump phosphorylated intermediates by electrophoresis. Anal. Biochem. 289, 267–273.
Richards, D. E., Rega, A. F., and Garrahan, P. J. (1978) Biochim. Biophys. Acta 51, 194–201.
Kratje, R. B., Garrahan, P. J., and Rega, A. F. (1983) Biochim. Biophys. Acta 731, 40–46.
Caride, A. J., Filoteo, A. G., Enyedi, A., Verma, A. K., and Penniston, J. T. (1996) Detection of isoform 4 of the plasma membrane calcium pump in human tissues by using isoform-specific monoclonal antibodies. Biochem. J. 31, 353–359.
Fraser, R. D. B. and Suzuki, E. In Physical Principles and Techniques of Protein Chemistry Part C (Leach, S. J., ed.). Academic Press, New York, 1973, pp. 301–335.
Akaike, H. (1974) A new look at the statistical model identification. IEEE Trans. Automatic Control 19, 716–723.
Sarkadi, B., Enyedi, A., Penniston, J. T., Verma, A. K., Dux, L., Molnár, E., and Gárdos, G. (1988) Characterization of membrane calcium pumps by simultaneous immunobloting and 32P radiography. Biochim. Biophys. Acta 939, 40–46.
Hellman, B., Gylfe, E., Grapeniesser, E., Lund, P. E., and Berts, A. (1992) Cytoplasmic Ca2+ oscillations in Pancreatic B-cells. Biochim. Biophys. Acta 1113, 295–305.
Ximenes, H. M., Kamagate, A., Van Eylen, F., Carpinelli, A., and Herchuelz, A. (2003) Opposite effects of glucose on plasma membrane Ca2+-ATPase and Na/Ca exchanger transcription, expression, and activity in rat pancreatic beta-cells. J. Biol. Chem. 278, 22956–22963.
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Rossi, J.P.F.C., Villamil, A.M., Echarte, M.M. et al. Plasma membrane calcium pump activity in rat pancreatic islets. Cell Biochem Biophys 46, 193–200 (2006). https://doi.org/10.1385/CBB:46:3:193
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DOI: https://doi.org/10.1385/CBB:46:3:193